Zhang Shen, Wu Yonggen, Weng Yimin, Xu Zhihui, Chen Wenmin, Zheng Dahan, Lin Wei, Liu Jun, Zhou Ying
Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University.
Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University.
J Vis Exp. 2017 Dec 17(130):55641. doi: 10.3791/55641.
14 day-old mouse ovarian tissue and preantral follicles isolated from same-aged mice were incubated in a simulated microgravity culture system. We quantitatively assessed follicle survival, measured follicle and oocyte diameters, and examined ultrastructure of the oocytes produced from the system. We observed decreased follicle survival, downregulation of expressions of proliferating cell nuclear antigen and growth differentiation factor 9, as indicators for the development of granulosa cells and oocytes, respectively, and oocyte ultrastructural abnormalities under the simulated microgravity condition. The simulated microgravity experimental setup needs to be optimized to provide a model for investigation of the mechanisms involved in the oocyte/follicle in vitro development.
将14日龄小鼠的卵巢组织和从同龄小鼠分离出的窦前卵泡在模拟微重力培养系统中进行培养。我们定量评估了卵泡存活率，测量了卵泡和卵母细胞的直径，并检查了该系统产生的卵母细胞的超微结构。我们观察到，在模拟微重力条件下，卵泡存活率降低，作为颗粒细胞和卵母细胞发育指标的增殖细胞核抗原和生长分化因子9的表达下调，以及卵母细胞超微结构异常。需要优化模拟微重力实验装置，以提供一个用于研究卵母细胞/卵泡体外发育所涉及机制的模型。
