Russu I Z, Rodionova N K, Bilko D I, Bilko N M
National University «Kyiv Mohyla Academy», Skovorody Str., 2, Kyiv, 04655 Ukraine.
Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Vasylkivska Str., 45, Kyiv, 03022 Ukraine.
Probl Radiac Med Radiobiol. 2017 Dec;22:224-230.
Under the influence of ionizing radiation on hematopoietic system, the level of its injury is determined not only by the radiosensitivity of hematopoietic stem cells, but also by radiation induced changes in microenvironment func tioning, in particular, mesenchymal stem cells as its components.
to define functioning characteristics of mesenchymal stem and progenitor cells of rats' bone marrow under prolonged action of ionizing radiation as a result of 90Sr incorporation.
We applied the model of Wistar rats' internal irradiation with 90Sr radionuclide and per formed the in vitro cultivation of their bone marrow mesenchymal cells. Colony forming efficiency in the in vitro cell culture was determined, as well as the possibility of these cells to form feeder layers and to support rat bone mar row hematopoietic cells in the culture of diffusion chambers in vitro.
We established that chronic action of incorporated 90Sr radionuclide induced considerable decrease in proliferative activity of mesenchymal stem cells comparing to control, as well as the inhibition of the capability to prolonged support of hematopoietic processes in vitro by their feeder layers.Thus, bone marrow mesenchymal stem cells and their closest progeny - progenitor cells were characterized by rather high radiosensitivity under the influence of ionizing radiation, which was revealed in considerable decline of their functional activity in cell culture in vitro comparing to control indices as a result of irradiation.
在电离辐射对造血系统的影响下，其损伤程度不仅取决于造血干细胞的放射敏感性，还取决于辐射诱导的微环境功能变化，特别是作为其组成部分的间充质干细胞。
确定由于90Sr掺入，电离辐射长期作用下大鼠骨髓间充质干细胞和祖细胞的功能特征。
我们应用90Sr放射性核素对Wistar大鼠进行体内照射模型，并对其骨髓间充质细胞进行体外培养。测定体外细胞培养中的集落形成效率，以及这些细胞在体外扩散室培养中形成饲养层并支持大鼠骨髓造血细胞的可能性。
我们确定，与对照相比，掺入的90Sr放射性核素的慢性作用导致间充质干细胞的增殖活性显著降低，以及其饲养层在体外长期支持造血过程的能力受到抑制。因此，骨髓间充质干细胞及其最接近的后代——祖细胞在电离辐射的影响下具有相当高的放射敏感性，这表现为与对照指标相比，由于辐射，它们在体外细胞培养中的功能活性显著下降。
