National University of Kyiv!Mohyla Academy, 2 Grygoriya Skovorody St., Kyiv, 04070, Ukraine.
Probl Radiac Med Radiobiol. 2023 Dec;28:216-224. doi: 10.33145/2304-8336-2023-28-216-224.
determination of the content of hematopoietic progenitor cells circulating in peripheral blood of Balb/Cmice, under ionizing radiation action in sublethal dose, at different periods after the irradiation, using cell culturein diffusion chambers in vivo.
Peripheral blood smears of Balb/C mice were prepared and studied, its cellular composition was determined, as well as by cultivation of peripheral blood cells in diffusion chambers in vivo their colony-forming efficien-cy was determined on the 0th, 5th, and 30th day after external irradiation in sublethal dose 5.85 Gy.
The content of myelocytes and metamyelocytes among blood nucleated cells of the irradiated animals wasincreased, compared to control, during the whole investigated period. In particular, on the 30th day after irradiationthe content of myelocytes in peripheral blood was 3.3 ± 0.7 % compared to (0.8 ± 0.4) % in control, and the content of metamyelocytes - (3.4 ± 0.7) % compared to (0.9 ± 0.3) % in control. A significant increase in the amountof circulating progenitor cells in the peripheral blood was observed in the early stages after irradiation (12.5 ± 1.6colony-forming units per 100,000 explanted cells, compared to 5.1 ± 0.8 in control). However, on the 5th day theircontent was slightly reduced compared to control (1.3 ± 0.9), and only to the 30th day a normalization of the amountof progenitor cells occurred in the peripheral blood (6.8 ± 0.7 colony-forming units per 100,000 explanted cells).
The analysis of the obtained results revealed an increased level of immature forms of cells in theperipheral blood of irradiated animals, compared to control, in the early stages after irradiation, includinghematopoietic progenitor cells, which are able to colony forming in cell culture. Therefore, the action of ionizingradiation in sublethal dose had a critical effect on the proliferation of hematopoietic cells in bone marrow and provoked their increased migration into the bloodstream. Determination of the content of hematopoietic cells' immature forms in peripheral blood allowed assessing the degree of hematopoietic damage due to the action of ionizing radiation.
在亚致死剂量电离辐射作用下，不同时期检测 Balb/C 小鼠外周血循环造血祖细胞含量，采用体内扩散室细胞培养法。
制备和研究 Balb/C 小鼠外周血涂片，确定其细胞组成，以及在 5.85 Gy 亚致死剂量外部照射后第 0、5 和 30 天，通过体内扩散室培养外周血细胞，确定其集落形成效率。
与对照组相比，照射动物血液有核细胞中的中幼粒细胞和晚幼粒细胞含量增加，在整个研究期间均如此。特别是在照射后第 30 天，外周血中中幼粒细胞含量为 3.3 ± 0.7%，而对照组为 0.8 ± 0.4%，晚幼粒细胞含量为 (3.4 ± 0.7)%，而对照组为 (0.9 ± 0.3)%。照射后早期观察到外周血中循环祖细胞数量显著增加（每 10 万个接种细胞 12.5 ± 1.6 个集落形成单位，而对照组为 5.1 ± 0.8）。然而，在第 5 天，其含量与对照组相比略有减少（1.3 ± 0.9），仅在第 30 天，外周血中祖细胞数量才恢复正常（每 10 万个接种细胞 6.8 ± 0.7 个集落形成单位）。
与对照组相比，照射后早期动物外周血中未成熟细胞水平升高，包括能够在细胞培养中形成集落的造血祖细胞。因此，亚致死剂量电离辐射作用对骨髓造血细胞的增殖具有关键影响，并促使其向血流中迁移增加。外周血中未成熟造血细胞含量的测定可评估电离辐射作用所致造血损伤的程度。
