Ahmadi Shahrzad, Goudarzi Hossein, Jalilvand Ahmad, Esmaeilzadeh Abdolreza
Razi Vaccine and Serum Research Institute, Karaj, Alborz. Email:
Asian Pac J Cancer Prev. 2017 Dec 29;18(12):3373-3377. doi: 10.22034/APJCP.2017.18.12.3373.
Objective: Cervical cancer is one of the most common cancers among women all over the world, and main cause is persistent infection with high risk human papillomavirus (HPV) strains. It has been reported that the distribution and prevalence of HPV types varies by geographical region, so that this is important for prevention by type-specific vaccines. The aim of current study was to determine the genotype distribution of HPV using the INNO-LiPA genotyping assay in Zanjan province, North West Iran. Methods: A total of 112 formalin-fixed paraffin embedded (FFPE) tissue samples from cases of low-grade intraepithelial lesion (LSIL), high-grade intraepithelial lesion (HSIL) and squamous cell carcinoma (SCC) were collected. The polymerase chain reaction (PCR) was used to amplify DNA for genotyping. Results: Among the 112 samples from females (ranging from 20 to 69 years, mean age 43.8 ± 10.1) tested for HPV DNA, 50 samples were positive. Based on results of genotyping, most common HPV genotypes were HPV18 (48%) followed by HPV-6 (24%), HPV73 (16%), HPV-51(8%), HPV-31(8%), HPV-16 (8%), HPV-56 (4%), HPV-44 (4%). Conclusion: While HPV infection is the major etiological factor for cervical cancer, presence was relatively low in our survey. In the positive cases, however, HPV18 was the most common in line with many other populations. The fact that types vary among different populations must clearly be taken into account in design of vaccines for our country.
宫颈癌是全球女性中最常见的癌症之一，主要病因是高危型人乳头瘤病毒（HPV）毒株的持续感染。据报道，HPV类型的分布和流行率因地理区域而异，因此这对于使用型特异性疫苗进行预防很重要。本研究的目的是使用INNO-LiPA基因分型检测法确定伊朗西北部赞詹省HPV的基因型分布。方法：共收集了112例来自低级别上皮内瘤变（LSIL）、高级别上皮内瘤变（HSIL）和鳞状细胞癌（SCC）病例的福尔马林固定石蜡包埋（FFPE）组织样本。采用聚合酶链反应（PCR）扩增DNA进行基因分型。结果：在检测HPV DNA的112例女性样本（年龄范围为20至69岁，平均年龄43.8±10.1）中，50例样本呈阳性。根据基因分型结果，最常见的HPV基因型是HPV18（48%），其次是HPV-6（24%）、HPV73（16%）、HPV-51（8%）、HPV-31（8%）、HPV-16（8%）、HPV-5&（4%）、HPV-44（4%）。结论：虽然HPV感染是宫颈癌的主要病因，但在我们的调查中其感染率相对较低。然而，在阳性病例中，HPV18与许多其他人群一样是最常见的。在为我国设计疫苗时，必须明确考虑到不同人群中HPV类型存在差异这一事实。
