Department of Agroecology, Research Center Flakkebjerg, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark.
Department of Molecular Biology and Genetics, Research Center Flakkebjerg, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark.
Chemosphere. 2018 Mar;195:624-631. doi: 10.1016/j.chemosphere.2017.12.121. Epub 2017 Dec 27.
Hairy roots induced by Agrobacterium rhizogenes are well established models to study the metabolism of xenobiotics in plants for phytoremediation purposes. However, the model requires special skills and resources for growing and is a time-consuming process. The roots induction process alters the genetic construct of a plant and is known to express genes that are normally absent from the non-transgenic plants. In this study, we propose and establish a non-transgenic maize root model to study xenobiotic metabolism in plants for phytoremediation purpose using azoxystrobin as a xenobiotic compound. Maize roots were grown aseptically in Murashige and Skoog medium for two weeks and were incubated in 100 μM azoxystrobin solution. Azoxystrobin was taken up by the roots to the highest concentration within 15 min of treatment and its phase I metabolites were also detected at the same time. Conjugated metabolites of azoxystrobin were detected and their identities were confirmed by enzymatic and mass spectrometric methods. Further, azoxystrobin metabolites identified in maize root culture were compared against azoxystrobin metabolites in azoxystrobin sprayed lettuce grown in green house. A very close similarity between metabolites identified in maize root culture and lettuce plant was obtained. The results from this study establish that non-transgenic maize roots can be used for xenobiotic metabolism studies instead of genetically transformed hairy roots due to the ease of growing and handling.
发根农杆菌诱导的毛状根是研究植物中异生物质代谢的良好模型，用于植物修复。然而，该模型需要特殊的技能和资源来进行培养，并且是一个耗时的过程。发根诱导过程改变了植物的遗传结构，已知会表达通常不存在于非转基因植物中的基因。在这项研究中，我们提出并建立了一个非转基因玉米根模型，用于使用肟菌酯作为异生物质化合物来研究植物中的异生物质代谢，用于植物修复。玉米根在无菌条件下在 Murashige 和 Skoog 培养基中培养两周，然后在 100μM 肟菌酯溶液中孵育。肟菌酯在处理后 15 分钟内被根吸收到最高浓度，同时也检测到其 I 相代谢物。通过酶和质谱方法检测到肟菌酯的结合代谢物，并确认了它们的身份。此外，通过比较温室中喷洒肟菌酯的生菜和玉米根培养物中鉴定的肟菌酯代谢物，发现它们非常相似。这项研究的结果表明，由于易于生长和处理，非转基因玉米根可以用于异生物质代谢研究，而不是遗传转化的毛状根。
