College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, PR China; Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing 400715, PR China.
Hunan Traditional Chinese Medical College, Zhuzhou 4120212, PR China.
Food Chem. 2018 Apr 15;245:1113-1123. doi: 10.1016/j.foodchem.2017.11.059. Epub 2017 Nov 16.
In this study, an efficient accelerated solvent extraction (ASE) technology was applied for rapid extraction of polysaccharides from the processing by-products of Chimonobambusa quadrangularis (CPS). The extraction yields, physicochemical characterization, and antioxidant activities of CPS obtained by ASE and hot water extraction (HWE) were further compared. A maximal ASE-CPS yield was obtained by optimized extraction conditions (temperature 126 °C, 2 cycles, and 22 min) using response surface methodology. The yield of polysaccharides from ASE (9.96% ± 0.39%) was significantly higher than that from HWE (7.16% ± 0.32%). Differences were found between ASE and HWE with the chemical composition, molecular weight distribution, rheological property, and antioxidant activities of the obtained polysaccharides, while the primary structure remained the same. ASE-CPS exhibited better chemical antioxidant activities in oxygen radical absorbance capacity (ORAC), reducing power, and DPPH and hydroxyl radical scavenging ability, whereas HWE-CPS displayed higher activity in metal chelating activity assay.
在这项研究中，采用高效加速溶剂萃取（ASE）技术快速提取慈竹加工副产物中的多糖。进一步比较了 ASE 和热水提取（HWE）得到的 CPS 的提取率、理化特性和抗氧化活性。通过响应面法优化提取条件（温度 126°C，2 个循环，22 分钟），获得了最大的 ASE-CPS 产率。ASE（9.96%±0.39%）的多糖产率明显高于 HWE（7.16%±0.32%）。ASE 和 HWE 之间在获得的多糖的化学成分、分子量分布、流变性能和抗氧化活性方面存在差异，而其一级结构保持不变。ASE-CPS 在氧自由基吸收能力（ORAC）、还原能力、DPPH 和羟基自由基清除能力方面表现出更好的化学抗氧化活性，而 HWE-CPS 在金属螯合活性测定中表现出更高的活性。
