Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain.
Food Chem. 2018 Apr 15;245:189-195. doi: 10.1016/j.foodchem.2017.10.041. Epub 2017 Oct 12.
A fast and simple analytical method was developed and characterized for the determination of aflatoxins (B, B, G and G) in rice. The procedure is based on a simple solid-liquid extraction without further clean-up, and analysis by ultra-high performance liquid chromatography coupled with fluorescence detection. Fluorescence emission of aflatoxins B and G was enhanced by post-column chemical derivatization using pyridinium bromide perbromide. The analytical method was satisfactorily characterized in white and brown rice. Under optimum conditions, external calibration in solvent could be used for quantification purposes and limits of quantification were below the maximum contents established by the European Union regulation for these contaminants/commodity group combination (0.07-0.14 µg/kg for white rice and 0.20-0.28 µg/kg for brown rice). Recovery studies carried out at three different concentration levels (0.5, 2 and 5 µg/kg) showed values in the range of 84.5-105.3%, and RSDs ≤ 5%.
建立并表征了一种快速、简单的分析方法，用于测定大米中的黄曲霉毒素（B1、B2、G1 和 G2）。该方法基于简单的固液萃取，无需进一步净化，通过超高效液相色谱-荧光检测进行分析。黄曲霉毒素 B1 和 G1 的荧光发射通过柱后化学衍生化用吡啶翁高溴酸盐增强。该分析方法在白米和糙米中得到了满意的表征。在最佳条件下，可在外标溶剂中进行定量分析，定量限低于欧盟法规为该污染物/商品组组合规定的最高含量（白米为 0.07-0.14 µg/kg，糙米为 0.20-0.28 µg/kg）。在三个不同浓度水平（0.5、2 和 5 µg/kg）进行的回收研究表明，回收率在 84.5-105.3%范围内，RSDs≤5%。
