College of Food Science, Southwest University, Chongqing 400715, China.
Chongqing Animal Disease Prevention and Control Center, Chongqing 401120, China.
Biomolecules. 2020 Apr 9;10(4):575. doi: 10.3390/biom10040575.
Label selection is an essential procedure for improving the sensitivity of fluorescence immunochromatography assays (FICAs). Under optimum conditions, time-resolved fluorescent nanobeads (TRFN), quantum dots nanobeads (QB) and quantum dots (QD)-based immunochromatography assays (TRFN-FICA, QB-FICA and QD-FICA) were systematically and comprehensively compared for the quantitative detection of aflatoxin B (AFB) in six grains (corn, soybeans, sorghum, wheat, rice and oat). All three FICAs can be applied as rapid, cost-effective and convenient qualitative tools for onsite screening of AFB; TRFN-FICA exhibits the best performance with the least immune reagent consumption, shortest immunoassay duration and lowest limit of detection (LOD). The LODs for TRFN-FICA, QB-FICA and QD-FICA are 0.04, 0.30 and 0.80 μg kg in six grains, respectively. Recoveries range from 83.64% to 125.61% at fortified concentrations of LOD, 2LOD and 4LOD, with the coefficient of variation less than 10.0%. Analysis of 60 field grain samples by three FICAs is in accordance with that of LC-MS/MS, and TRFN-FICA obtained the best fit. In conclusion, TRFN-FICA is more suitable for quantitative detection of AFB in grains when the above factors are taken into consideration.
标签选择是提高荧光免疫层析分析（FICA）灵敏度的重要步骤。在最佳条件下，时间分辨荧光纳米珠（TRFN）、量子点纳米珠（QB）和量子点（QD）基于免疫层析分析（TRFN-FICA、QB-FICA 和 QD-FICA）被系统和全面地比较，用于定量检测六种谷物（玉米、大豆、高粱、小麦、大米和燕麦）中的黄曲霉毒素 B（AFB）。所有三种 FICA 都可以作为快速、经济高效和方便的现场筛选 AFB 的定性工具；TRFN-FICA 表现出最佳性能，免疫试剂消耗最少，免疫分析时间最短，检测限（LOD）最低。在六种谷物中，TRFN-FICA、QB-FICA 和 QD-FICA 的 LOD 分别为 0.04、0.30 和 0.80 μg kg。在 LOD、2LOD 和 4LOD 的加标浓度下，回收率范围为 83.64%至 125.61%，变异系数小于 10.0%。三种 FICA 对 60 个田间谷物样品的分析与 LC-MS/MS 一致，并且 TRFN-FICA 拟合效果最好。总之，考虑到上述因素，TRFN-FICA 更适合用于谷物中 AFB 的定量检测。
