变应性鼻炎小鼠lncRNA和mRNA表达谱的微阵列分析
Microarray analysis of lncRNA and mRNA expression profiles in mice with allergic rhinitis.
作者信息
Ma Yue, Shi Le, Zheng Chunquan
机构信息
Department of Otolaryngology-Head and Neck Surgery, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai Key Clinical Disciplines of Otorhinolaryngology, PR China.
Department of Otolaryngology-Head and Neck Surgery, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai Key Clinical Disciplines of Otorhinolaryngology, PR China.
出版信息
Int J Pediatr Otorhinolaryngol. 2018 Jan;104:58-65. doi: 10.1016/j.ijporl.2017.10.046. Epub 2017 Nov 7.
OBJECTIVES
We aimed to identify the effect of lncRNAs in CD4 T cells on Allergic rhinitis (AR).
METHODS
The present study conducted a microarray to identify the expression profiles of lncRNA and mRNA in CD4 T cells in both AR murine models and normal controls. And qRT-PCR was used to confirm the results. GO and KEGG enrichment analysis were used to show all related pathways and a co-expression network was conducted to find lncRNAs which have high correlation with these pathways.
RESULTS
The results showed that the two groups contained a total of 158 deregulated lncRNAs, of which 110 were upregulated and 48 were downregulated. And positive regulation of calcium ion transport, B cell activation, chemokine-signaling pathways and calcium-signaling pathways may be involved in the development of T cells in AR pathology. Finally, we can find the differentially expressed mRNA in the pathways related to T cell differentiation correlated with many deregulated lncRNAs.
CONCLUSIONS
The present study was the first to show the differential expression profiles of lncRNAs in the CD4 T cells of an AR murine model, which may provide significant insights into AR pathogenesis and offer new treatment targets to alleviate it.
目的
我们旨在确定CD4 T细胞中的长链非编码RNA（lncRNA）对变应性鼻炎（AR）的影响。
方法
本研究进行了一项微阵列分析，以确定AR小鼠模型和正常对照中CD4 T细胞中lncRNA和mRNA的表达谱。并使用qRT-PCR来证实结果。基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析用于展示所有相关途径，并构建共表达网络以找到与这些途径高度相关的lncRNA。
结果
结果显示，两组共有158个失调的lncRNA，其中110个上调，48个下调。钙离子转运的正调控、B细胞活化、趋化因子信号通路和钙信号通路可能参与了AR病理过程中T细胞的发育。最后，我们发现在与T细胞分化相关的途径中差异表达的mRNA与许多失调的lncRNA相关。
结论
本研究首次展示了AR小鼠模型CD4 T细胞中lncRNA的差异表达谱，这可能为AR的发病机制提供重要见解，并为缓解该病提供新的治疗靶点。
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