深入了解克氏锥虫病传播媒介——忽视罗蛉的唾液蛋白质组
A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.
作者信息
Santiago Paula Beatriz, Assumpção Teresa C F, de Araújo Carla Nunes, Bastos Izabela Marques Dourado, Neves David, da Silva Ionizete Garcia, Charneau Sébastien, Queiroz Rayner Myr L, Raiol Tainá, Oliveira João Victor de Araújo, de Sousa Marcelo Valle, Calvo Eric, Ribeiro José M C, Santana Jaime M
机构信息
Department of Cell Biology, The University of Brasília, Brasília, Brazil.
Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, Rockville, Maryland, United States of America.
出版信息
PLoS Negl Trop Dis. 2016 Apr 29;10(4):e0004581. doi: 10.1371/journal.pntd.0004581. eCollection 2016 Apr.
BACKGROUND
Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses.
METHODS/PRINCIPAL FINDINGS: Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant.
CONCLUSIONS/SIGNIFICANCE: The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts.
背景
锥蝽是吸血昆虫，是恰加斯病的传播媒介。忽视红猎蝽是其中一种被发现的猎蝽，对这种美洲锥虫病的传播有一定作用。吸血节肢动物的唾液中含有生物活性分子，可用于对抗宿主的止血、炎症和免疫反应。
方法/主要发现：采用下一代测序和基于质谱的蛋白质鉴定技术来研究忽视红猎蝽唾液的成分。我们向GenBank数据库存入了4230个编码DNA序列（CDS）。从组装好的 reads 中提取了一组636个推测具有分泌性质的蛋白质的CDS，其中73个通过蛋白质组学分析得到确认。对忽视红猎蝽的唾液蛋白质组进行了表征，并检测到丝氨酸蛋白酶转录本。揭示了一些普遍存在的蛋白质家族，包括脂钙蛋白、丝氨酸蛋白酶抑制剂和抗原5。金属蛋白酶、去整合素和气味结合蛋白家族的丰度较低。
结论/意义：所呈现的数据增进了我们对吸血节肢动物唾液蛋白质组的理解，并有助于理解吸血行为以及媒介与其脊椎动物宿主之间的复杂相互作用。
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