Barliana Melisa I, Amalya Shintya N, Pradipta Ivan S, Alfian Sofa D, Kusuma Arif Sw, Milanda Tiana, Abdulah Rizky
Department of Biological Pharmacy, Biotechnology Pharmacy Laboratory.
Pharmacy Services Development Research Center.
Psychol Res Behav Manag. 2017 Dec 15;10:395-401. doi: 10.2147/PRBM.S152451. eCollection 2017.
Daily life stress markedly affects the response toward stressful stimuli. DNA methy-lation is one of the factors that regulate this response, and is a normal mechanism of somatic cell growth, but its regulatory gene variations may cause alterations in the stress response. The aim of the present study was to investigate genotypic variants of the DNA methyltransferase 3A () gene in 129 healthy subjects and evaluate its association with daily life stress. Blood samples were collected, and genomic DNA was isolated. DNA was amplified using specific tetra primers for (C/T) rs11683424 and visualized following 2% agarose gel electrophoresis. The association of DNMT3A genetic variants with daily life stress was analyzed using the Kessler Psychological Distress Scale (K10). We observed that the distribution of subjects with genotype CC (wild type), CT (heteromutant), and TT (homomutant) was 13.95%, 81.4%, and 4.65%, respectively. Genetic variations significantly affected the daily life stress condition (=0.04) in Indonesian healthy subjects, but most of the subjects with the CT phenotype were classified in a stress condition.
日常生活压力显著影响对压力刺激的反应。DNA甲基化是调节这种反应的因素之一，是体细胞生长的正常机制，但其调控基因变异可能导致应激反应改变。本研究的目的是调查129名健康受试者中DNA甲基转移酶3A（）基因的基因型变异，并评估其与日常生活压力的关联。采集血样，分离基因组DNA。使用针对（C/T）rs11683424的特异性四引物扩增DNA，并在2%琼脂糖凝胶电泳后进行可视化。使用凯斯勒心理困扰量表（K10）分析DNMT3A基因变异与日常生活压力的关联。我们观察到，基因型CC（野生型）、CT（杂合突变型）和TT（纯合突变型）受试者的分布分别为13.95%、81.4%和4.65%。基因变异显著影响印度尼西亚健康受试者的日常生活压力状况（=0.04），但大多数CT表型的受试者被归类为处于压力状态。
