Nagendran K, Mohankumar S, Mohammed Faisal P, Bagewadi B, Karthikeyan G
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003 India.
Present Address: Division of Crop Protection, ICAR - Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh, 221005 India.
Virusdisease. 2017 Dec;28(4):425-429. doi: 10.1007/s13337-017-0403-7. Epub 2017 Oct 9.
During 2012-2014, mosaic disease on chayote in the farmers field of Kodaikanal region (high altitude zone) of Tamil Nadu was observed. The disease was characterized with severe mosaic, cupping and enation on leaves with reduced fruit size. Disease was found to causes an yield loss of more than 60% with the maximum disease incidence of 100% for the past 5 years consecutively. Preliminary serological and molecular screening indicated the association of begomovirus with the disease. Complete nucleotide sequence and phylogenetic analysis of DNA A revealed the identity of the virus as tomato leaf curl New Delhi virus (ToLCNDV). In recombination analysis study, the major parent was identified as ToLCNDV from Pakistan infecting tomato. Thus the present finding confirms expansion of new geographical region and host for ToLCNDV causing mosaic disease on chayote from Tamil Nadu. To our knowledge this is the first confirmed report for the occurrence of ToLCNDV on chayote in southern India.
2012年至2014年期间，在泰米尔纳德邦高海拔地区科代卡纳尔地区的农民田地里，佛手瓜上出现了花叶病。该病的特征是叶片严重花叶、杯状化和疱状突起，果实变小。发现该病导致产量损失超过60%，在过去连续5年中最高发病率达100%。初步血清学和分子筛查表明双生病毒与该病有关。DNA A的完整核苷酸序列和系统发育分析显示该病毒为番茄卷叶新德里病毒（ToLCNDV）。在重组分析研究中，主要亲本被鉴定为来自巴基斯坦感染番茄的ToLCNDV。因此，本研究结果证实了新的地理区域和宿主的扩展，即ToLCNDV在泰米尔纳德邦导致佛手瓜花叶病。据我们所知，这是印度南部首次关于ToLCNDV在佛手瓜上发生的确诊报告。
