Phaneendra Chigurupati, Rao K R S S, Jain R K, Mandal B
Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012 India.
Indian J Virol. 2012 Jun;23(1):42-5. doi: 10.1007/s13337-011-0054-z. Epub 2011 Dec 6.
During 2006, pumpkin leaf curl-a new disease was observed in the experimental field at Indian Agricultural Research Institute. The disease was characterized by upward leaf curl with chlorotic patches and stunting of plant. Polymerase chain reaction (PCR) with coat protein specific primers to Tomato leaf curl New Delhi virus (ToLCNDV) indicated association of a begomovirus with the disease. The sequence comparison and phylogenetic analysis of the complete DNA genome further revealed the identity of the virus as ToLCNDV. The study provides evidence that ToLCNDV is associated with the leaf curl of pumpkin (Cucurbita moschata) in northern India.
2006年期间，在印度农业研究所的试验田中发现了一种南瓜卷叶新病害。该病害的特征是叶片向上卷曲、出现褪绿斑以及植株矮化。用针对番茄卷叶新德里病毒（ToLCNDV）外壳蛋白的特异性引物进行聚合酶链反应（PCR），结果表明一种双生病毒与该病害有关。对完整DNA基因组进行序列比较和系统发育分析，进一步揭示该病毒为ToLCNDV。这项研究提供了证据，证明ToLCNDV与印度北部南瓜（南瓜属）的卷叶病有关。
