一种用于检测人类临床标本中鹦鹉热衣原体和肺炎衣原体的多重TaqMan实时荧光定量PCR检测方法的开发。
Development of a multiplex TaqMan real-time PCR assay for the detection of Chlamydia psittaci and Chlamydia pneumoniae in human clinical specimens.
作者信息
Wolff Bernard J, Morrison Shatavia S, Winchell Jonas M
机构信息
Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
出版信息
Diagn Microbiol Infect Dis. 2018 Mar;90(3):167-170. doi: 10.1016/j.diagmicrobio.2017.11.014. Epub 2017 Nov 27.
Diagnosis of Chlamydia psittaci and Chlamydia pneumoniae infections has traditionally relied on serological assays. We developed a multiplex real-time PCR assay for detection of C. psittaci, C. pneumoniae and an internal control. Results of this assay demonstrated 100% concordance compared to results of previously tested human clinical specimens.
鹦鹉热衣原体和肺炎衣原体感染的诊断传统上依赖于血清学检测。我们开发了一种用于检测鹦鹉热衣原体、肺炎衣原体和一个内部对照的多重实时聚合酶链反应检测法。该检测法的结果与先前检测的人类临床标本的结果相比显示出100%的一致性。
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