• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

缺氧脂肪细胞和肥胖个体来源的细胞外囊泡降低胰岛素刺激的葡萄糖摄取。

Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.

机构信息

Department of Molecular and Cellular Physiology, Institute of Translational Medicine, The University of Liverpool, Liverpool, UK.

CIC bioGUNE, CIBERehd, Derio, Bizkaia, Spain.

出版信息

Mol Nutr Food Res. 2018 Mar;62(5). doi: 10.1002/mnfr.201700917. Epub 2018 Feb 20.

DOI:10.1002/mnfr.201700917
PMID:29292863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5887919/
Abstract

SCOPE

We investigate the effects of extracellular vesicles (EVs) obtained from in vitro adipocyte cell models and from obese subjects on glucose transport and insulin responsiveness.

METHODS AND RESULTS

EVs are isolated from the culture supernatant of adipocytes cultured under normoxia, hypoxia (1% oxygen), or exposed to macrophage conditioned media (15% v/v). EVs are isolated from the plasma of lean individuals and subjects with obesity. Cultured adipocytes are incubated with EVs and activation of insulin signalling cascades and insulin-stimulated glucose transport are measured. EVs released from hypoxic adipocytes impair insulin-stimulated 2-deoxyglucose uptake and reduce insulin mediated phosphorylation of AKT. Insulin-mediated phosphorylation of extracellular regulated kinases (ERK1/2) is not affected. EVs from individuals with obesity decrease insulin stimulated 2-deoxyglucose uptake in adipocytes (p = 0.0159).

CONCLUSION

EVs released by stressed adipocytes impair insulin action in neighboring adipocytes.

摘要

范围

我们研究了体外脂肪细胞模型和肥胖受试者来源的细胞外囊泡（EVs）对葡萄糖转运和胰岛素反应的影响。

方法和结果

EVs 是从常氧、低氧（1%氧气）或巨噬细胞条件培养基（15%v/v）培养的脂肪细胞的培养上清液中分离得到的。EVs 是从瘦个体和肥胖个体的血浆中分离得到的。培养的脂肪细胞与 EVs 孵育，并测量胰岛素信号级联的激活和胰岛素刺激的葡萄糖转运。从低氧脂肪细胞释放的 EVs 损害胰岛素刺激的 2-脱氧葡萄糖摄取，并降低胰岛素介导的 AKT 磷酸化。胰岛素介导的细胞外调节激酶（ERK1/2）的磷酸化不受影响。肥胖个体来源的 EVs 降低了脂肪细胞中胰岛素刺激的 2-脱氧葡萄糖摄取（p = 0.0159）。

结论

应激脂肪细胞释放的 EVs 损害了邻近脂肪细胞中的胰岛素作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f93/5887919/f8a8ecd3f03b/MNFR-62-na-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f93/5887919/2b495d8ef61d/MNFR-62-na-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f93/5887919/a7ba18addc11/MNFR-62-na-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f93/5887919/f09651b500a8/MNFR-62-na-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f93/5887919/f8a8ecd3f03b/MNFR-62-na-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f93/5887919/2b495d8ef61d/MNFR-62-na-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f93/5887919/a7ba18addc11/MNFR-62-na-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f93/5887919/f09651b500a8/MNFR-62-na-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f93/5887919/f8a8ecd3f03b/MNFR-62-na-g004.jpg

相似文献

1
Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.缺氧脂肪细胞和肥胖个体来源的细胞外囊泡降低胰岛素刺激的葡萄糖摄取。
Mol Nutr Food Res. 2018 Mar;62(5). doi: 10.1002/mnfr.201700917. Epub 2018 Feb 20.
2
Impaired activation of protein kinase C-zeta by insulin and phosphatidylinositol-3,4,5-(PO4)3 in cultured preadipocyte-derived adipocytes and myotubes of obese subjects.在肥胖受试者培养的前脂肪细胞来源的脂肪细胞和肌管中，胰岛素和磷脂酰肌醇-3,4,5-三磷酸（PO4）3对蛋白激酶C-ζ的激活受损。
J Clin Endocrinol Metab. 2004 Aug;89(8):3994-8. doi: 10.1210/jc.2004-0106.
3
Circulating adipocyte-derived extracellular vesicles are novel markers of metabolic stress.循环脂肪细胞衍生的细胞外囊泡是代谢应激的新型标志物。
J Mol Med (Berl). 2016 Nov;94(11):1241-1253. doi: 10.1007/s00109-016-1446-8. Epub 2016 Jul 9.
4
Amino acids require glucose to enhance, through phosphoinositide-dependent protein kinase 1, the insulin-activated protein kinase B cascade in insulin-resistant rat adipocytes.氨基酸需要葡萄糖，通过磷酸肌醇依赖性蛋白激酶1来增强胰岛素抵抗大鼠脂肪细胞中胰岛素激活的蛋白激酶B级联反应。
Diabetologia. 2006 May;49(5):1017-26. doi: 10.1007/s00125-006-0201-z. Epub 2006 Mar 21.
5
Effect of conditioned media from mature human adipocytes on insulin-stimulated Akt/PKB phosphorylation in human skeletal muscle cells: role of BMI and fat cell size.成熟人类脂肪细胞的条件培养基对人骨骼肌细胞中胰岛素刺激的Akt/PKB磷酸化的影响：BMI和脂肪细胞大小的作用
Horm Metab Res. 2009 Mar;41(3):190-6. doi: 10.1055/s-0028-1093342. Epub 2008 Oct 27.
6
Overexpression of protein-tyrosine phosphatase-1B in adipocytes inhibits insulin-stimulated phosphoinositide 3-kinase activity without altering glucose transport or Akt/Protein kinase B activation.脂肪细胞中蛋白酪氨酸磷酸酶-1B的过表达会抑制胰岛素刺激的磷酸肌醇3激酶活性，而不会改变葡萄糖转运或Akt/蛋白激酶B的激活。
J Biol Chem. 2000 Jun 16;275(24):18318-26. doi: 10.1074/jbc.M908392199.
7
Comparative and functional analysis of plasma membrane-derived extracellular vesicles from obese vs. nonobese women.肥胖女性与非肥胖女性血浆膜衍生细胞外囊泡的比较和功能分析。
Clin Nutr. 2020 Apr;39(4):1067-1076. doi: 10.1016/j.clnu.2019.04.008. Epub 2019 Apr 17.
8
An inhibitor of p38 mitogen-activated protein kinase prevents insulin-stimulated glucose transport but not glucose transporter translocation in 3T3-L1 adipocytes and L6 myotubes.p38丝裂原活化蛋白激酶抑制剂可阻止3T3-L1脂肪细胞和L6肌管中胰岛素刺激的葡萄糖转运，但不影响葡萄糖转运体的易位。
J Biol Chem. 1999 Apr 9;274(15):10071-8. doi: 10.1074/jbc.274.15.10071.
9
A novel pleckstrin homology domain-containing protein enhances insulin-stimulated Akt phosphorylation and GLUT4 translocation in adipocytes.一种新型含 PH 结构域蛋白增强了脂肪细胞中胰岛素刺激的 Akt 磷酸化和 GLUT4 转位。
J Biol Chem. 2010 Sep 3;285(36):27581-9. doi: 10.1074/jbc.M110.146886. Epub 2010 Jun 28.
10
Insulin acutely increases glucose transporter 1 on plasma membranes and glucose uptake in an AKT-dependent manner in chicken adipocytes.胰岛素在鸡脂肪细胞中以 AKT 依赖的方式使质膜上的葡萄糖转运蛋白 1 急性增加和葡萄糖摄取。
Gen Comp Endocrinol. 2019 Nov 1;283:113232. doi: 10.1016/j.ygcen.2019.113232. Epub 2019 Jul 26.

引用本文的文献

1
Adipose tissue dysfunction disrupts metabolic homeostasis: mechanisms linking fat dysregulation to disease.脂肪组织功能障碍破坏代谢稳态：将脂肪调节异常与疾病联系起来的机制。
Front Endocrinol (Lausanne). 2025 Jun 24;16:1592683. doi: 10.3389/fendo.2025.1592683. eCollection 2025.
2
Obesity and Adipose-Derived Extracellular Vesicles: Implications for Metabolic Regulation and Disease.肥胖与脂肪源性细胞外囊泡：对代谢调节和疾病的影响
Biomolecules. 2025 Feb 5;15(2):231. doi: 10.3390/biom15020231.
3
Treating Metabolic Dysregulation and Senescence by Caloric Restriction: Killing Two Birds with One Stone?

本文引用的文献

1
Extracellular Vesicles: Novel Mediators of Cell Communication In Metabolic Disease.细胞外囊泡：代谢疾病中细胞通讯的新型介质。
Trends Endocrinol Metab. 2017 Jan;28(1):3-18. doi: 10.1016/j.tem.2016.10.003. Epub 2016 Oct 31.
2
Circulating adipocyte-derived extracellular vesicles are novel markers of metabolic stress.循环脂肪细胞衍生的细胞外囊泡是代谢应激的新型标志物。
J Mol Med (Berl). 2016 Nov;94(11):1241-1253. doi: 10.1007/s00109-016-1446-8. Epub 2016 Jul 9.
3
Characterisation of adipocyte-derived extracellular vesicles released pre- and post-adipogenesis.
通过热量限制治疗代谢失调和衰老：一石二鸟？
Antioxidants (Basel). 2025 Jan 16;14(1):99. doi: 10.3390/antiox14010099.
4
Circulating extracellular vesicle-carried PTP1B and PP2A phosphatases as regulators of insulin resistance.循环细胞外囊泡携带的蛋白酪氨酸磷酸酶1B和蛋白磷酸酶2A作为胰岛素抵抗的调节因子
Diabetologia. 2025 Jan;68(1):231-242. doi: 10.1007/s00125-024-06288-0. Epub 2024 Oct 18.
5
Adipokines in the Crosstalk between Adipose Tissues and Other Organs: Implications in Cardiometabolic Diseases.脂肪组织与其他器官相互作用中的脂肪因子：对心血管代谢疾病的影响
Biomedicines. 2024 Sep 19;12(9):2129. doi: 10.3390/biomedicines12092129.
6
MicroRNAs as Epigenetic Regulators of Obesity.微小 RNA 作为肥胖的表观遗传调控因子。
Adv Exp Med Biol. 2024;1460:595-627. doi: 10.1007/978-3-031-63657-8_20.
7
Cellular Senescence and Extracellular Vesicles in the Pathogenesis and Treatment of Obesity-A Narrative Review.细胞衰老与细胞外囊泡在肥胖发病机制及治疗中的作用：综述
Int J Mol Sci. 2024 Jul 20;25(14):7943. doi: 10.3390/ijms25147943.
8
Adipose Tissue Dysfunction and the Role of Adipocyte-Derived Extracellular Vesicles in Obesity and Metabolic Syndrome.脂肪组织功能障碍以及脂肪细胞衍生的细胞外囊泡在肥胖和代谢综合征中的作用。
J Endocr Soc. 2024 Jun 25;8(8):bvae126. doi: 10.1210/jendso/bvae126. eCollection 2024 Jul 1.
9
Increased secretion of adipocyte-derived extracellular vesicles is associated with adipose tissue inflammation and the mobilization of excess lipid in human obesity.脂肪细胞衍生的细胞外囊泡的分泌增加与人类肥胖症中脂肪组织的炎症和多余脂质的动员有关。
J Transl Med. 2024 Jul 4;22(1):623. doi: 10.1186/s12967-024-05249-w.
10
Extracellular vesicles released by steatotic hepatocytes alter adipocyte metabolism.脂肪变性的肝细胞释放的细胞外囊泡会改变脂肪细胞的代谢。
J Extracell Biol. 2022 Mar 15;1(2):e32. doi: 10.1002/jex2.32. eCollection 2022 Feb.
前脂肪细胞和脂肪细胞分化后释放的脂肪细胞衍生细胞外囊泡的特征。
J Extracell Vesicles. 2015 Nov 24;4:29159. doi: 10.3402/jev.v4.29159. eCollection 2015.
4
Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.大冢长- Evans 德岛肥胖（OLETF）大鼠脂肪细胞释放的细胞外囊泡的蛋白质组学分析
Protein J. 2015 Jun;34(3):220-35. doi: 10.1007/s10930-015-9616-z.
5
Biological properties of extracellular vesicles and their physiological functions.细胞外囊泡的生物学特性及其生理功能。
J Extracell Vesicles. 2015 May 14;4:27066. doi: 10.3402/jev.v4.27066. eCollection 2015.
6
Cell-derived extracellular vesicles as a platform to identify low-invasive disease biomarkers.细胞衍生的细胞外囊泡作为一种平台，用于识别低侵入性疾病生物标志物。
Expert Rev Mol Diagn. 2015;15(7):907-23. doi: 10.1586/14737159.2015.1043272. Epub 2015 May 6.
7
Hypoxia upregulates Rab11-family interacting protein 4 through HIF-1α to promote the metastasis of hepatocellular carcinoma.缺氧通过 HIF-1α 上调 Rab11 家族相互作用蛋白 4 以促进肝细胞癌的转移。
Oncogene. 2015 Dec 3;34(49):6007-17. doi: 10.1038/onc.2015.49. Epub 2015 Mar 9.
8
Effect of extracellular vesicles of human adipose tissue on insulin signaling in liver and muscle cells.人脂肪组织细胞外囊泡对肝和肌细胞胰岛素信号的影响。
Obesity (Silver Spring). 2014 Oct;22(10):2216-23. doi: 10.1002/oby.20847. Epub 2014 Jul 17.
9
Adiponectin is partially associated with exosomes in mouse serum.脂联素部分与小鼠血清中的外泌体相关。
Biochem Biophys Res Commun. 2014 Jun 6;448(3):261-6. doi: 10.1016/j.bbrc.2014.04.114. Epub 2014 May 2.
10
Quantitative proteomic analysis of hepatocyte-secreted extracellular vesicles reveals candidate markers for liver toxicity.肝细胞分泌的细胞外囊泡的定量蛋白质组学分析揭示了肝毒性的候选标志物。
J Proteomics. 2014 May 30;103:227-40. doi: 10.1016/j.jprot.2014.04.008. Epub 2014 Apr 18.