Keiderling Timothy A, Lakhani Ahmed
Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois, USA.
Department of Biophysical Chemistry and Mathematics, Calumet College of St. Joseph, Whiting, Indiana, USA.
Chirality. 2018 Mar;30(3):238-253. doi: 10.1002/chir.22799. Epub 2018 Jan 2.
Vibrational circular dichroism (VCD) has become a standard method for determination of absolute stereochemistry, particularly now that reliable commercial instrumentation has become available. These instruments use a now well-documented Fourier transform infrared-based approach to measure VCD that has virtually displaced initial dispersive infrared-based designs. Nonetheless, many papers have appeared reporting dispersive VCD data, especially for biopolymers. Instrumentation designed with these original methods, particularly after more recent updates optimizing performance in selected spectral regions, has been shown still to have advantages for specific applications. This article presents a mini-review of dispersive VCD instrument designs and includes sample spectra obtained for various biopolymer (particularly peptide) samples. Complementary reviews of Fourier transform-VCD designs are broadly available.
振动圆二色性（VCD）已成为确定绝对立体化学的标准方法，尤其是现在已有可靠的商用仪器。这些仪器采用一种现已记录详尽的基于傅里叶变换红外的方法来测量VCD，这种方法实际上已经取代了最初基于色散红外的设计。尽管如此，仍有许多论文报道了色散VCD数据，特别是关于生物聚合物的。事实证明，采用这些原始方法设计的仪器，尤其是在经过最近的更新以优化选定光谱区域的性能之后，在特定应用中仍具有优势。本文对色散VCD仪器设计进行了简要综述，并展示了各种生物聚合物（特别是肽）样品的光谱。关于傅里叶变换VCD设计的补充综述广泛可得。
