Wei Yongqin, Vriesekoop Frank, Yuan Qipeng, Liang Hao
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing100029, P. R. China.
Department of Food Science, Harper Adams University, Newport TF10 8NB, Shropshire, England.
ACS Omega. 2018 Sep 28;3(9):12246-12252. doi: 10.1021/acsomega.8b01576. eCollection 2018 Sep 30.
Based on the fact that β-lactoglobulin (β-lg) can solubilize readily in water and bind many small hydrophobic molecules, a novel nanocomplexed glabridin with β-lg was developed by an antisolvent precipitation method. After binding to β-lg, the solubility of glabridin in aqueous solution was enhanced 21 times. Fluorescence spectroscopy of β-lg revealed that the interaction of glabridin with β-lg made the environment of Trp and Tyr residues on β-lg more hydrophilic. The morphology and crystal form of the nanocomplexed glabridin with β-lg was characterized and the changes in β-lg conformation was also been investigated. In combination with molecular docking modeling, the results revealed that glabridin was bound to β-lg by hydrophobic forces and hydrogen-bond interactions. Furthermore, the nanocomplexed glabridin with β-lg had a better 2,2-diphenyl-1-picrylhydrazyl radical-scavenging capacity and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid radical-scavenging capacity compared to free glabridin at the same concentration during in vitro tests. Thus, nanocomplexing with β-lg, by virtue of its ability to enhance the solubility of glabridin in aqueous systems, provides a suitable opportunity as a nanocarrier molecule.
基于β-乳球蛋白（β-lg）能轻易溶于水并结合许多小的疏水分子这一事实，通过反溶剂沉淀法制备了一种新型的与β-lg复合的光甘草定纳米复合物。与β-lg结合后，光甘草定在水溶液中的溶解度提高了21倍。β-lg的荧光光谱表明，光甘草定与β-lg的相互作用使β-lg上色氨酸和酪氨酸残基的环境更具亲水性。对与β-lg复合的光甘草定纳米复合物的形态和晶型进行了表征，并研究了β-lg构象的变化。结合分子对接模型，结果表明光甘草定通过疏水力和氢键相互作用与β-lg结合。此外，在体外试验中，与相同浓度的游离光甘草定相比，与β-lg复合的光甘草定纳米复合物具有更好的2,2-二苯基-1-苦基肼自由基清除能力和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸自由基清除能力。因此，与β-lg复合，凭借其提高光甘草定在水性体系中溶解度的能力，为作为纳米载体分子提供了一个合适的契机。
