Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; Key Laboratory of Zoonosis of Ministry of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; Key Laboratory of Zoonosis of Ministry of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
Food Chem Toxicol. 2018 Feb;112:310-319. doi: 10.1016/j.fct.2017.12.066. Epub 2017 Dec 30.
Trichothecene mycotoxins are a group of structurally related sesquiterpenoid metabolites produced by multiple Fusarium species that often contaminate cereals and threaten human and animal health. The basic structure of this mycotoxin group is a characteristic 12, 13-epoxide group, which is considered an essential functional group for toxicity. In this study, using trichothecene mycotoxin deoxynivalenol (DON) as a representative substrate, we identified a novel trichothecene deepoxidation bacterium, Eggerthella sp. DII-9 (DII-9), from chicken intestines. DII-9 can grow and transform DON over abroad range of temperatures (20-45 °C) and pH values (5-10), suggesting the possibility of developing promising future applications as feed additives. Substrate specificity analysis showed that DII-9 is capable of promoting the deepoxidation of DON, HT-2, T-2 triol and T-2 tetraol. To explore the molecular mechanisms of the de-epoxidation of trichothecenes, the complete genome of DII-9 was sequenced and characterized. Altogether, a novel detoxification bacterium for trichothecene mycotoxins was identified and characterized.
三脱氧镰刀菌烯醇类真菌毒素是一组结构相关的倍半萜烯代谢物，由多种镰刀菌属产生，常污染谷物并威胁人类和动物健康。该真菌毒素组的基本结构是特征性的 12、13-环氧化物基团，被认为是毒性的必需功能基团。在这项研究中，我们使用三脱氧镰刀菌烯醇类真菌毒素脱氧雪腐镰刀菌烯醇（DON）作为代表性底物，从鸡肠道中鉴定出一种新型三脱氧镰刀菌深层氧化细菌，即 Eggerthella sp. DII-9（DII-9）。DII-9 可以在较宽的温度（20-45°C）和 pH 值（5-10）范围内生长和转化 DON，这表明其作为饲料添加剂具有很大的应用潜力。底物特异性分析表明，DII-9 能够促进 DON、HT-2、T-2 三醇和 T-2 四醇的深层氧化。为了探索三脱氧镰刀菌烯醇去环氧化的分子机制，我们对 DII-9 的完整基因组进行了测序和特征分析。总之，我们鉴定并表征了一种新型的三脱氧镰刀菌烯醇真菌毒素解毒细菌。
