Liu Yanrong, Ma Mingxin, Tang Yu, Huang Zhenqian, Guo Yongpeng, Ma Qiugang, Zhao Lihong
State Key Laboratory of Animal Nutrition and Feeding, Poultry Nutrition and Feed Technology Innovation Team, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Foods. 2024 Mar 29;13(7):1064. doi: 10.3390/foods13071064.
Deoxynivalenol (DON), primarily generated by species, often exists in agricultural products. It can be transformed to 3--deoxynivalenol (3--DON), with a relatively low toxicity, via two steps. DDH in ANSP101 was proved to convert DON to 3-keto-deoxynivalenol (3-keto-DON). In the present research, AKR4, a NADPH-dependent aldo/keto reductase from ANSP101, was identified to be capable of converting 3-keto-DON into 3--DON. Our results demonstrated that AKR4 is clearly a NADPH-dependent enzyme, for its utilization of NADPH is higher than that of NADH. AKR4 functions at a range of pH 5-10 and temperatures of 20-60 °C. AKR4 is able to degrade 89% of 3-keto-DON in 90 min at pH 7 and 50 °C with NADPH as the cofactor. The discovery of AKR4, serving as an enzyme involved in the final step in DON degradation, might provide an option for the final detoxification of DON in food and feed.
脱氧雪腐镰刀菌烯醇（DON）主要由某些物种产生，常存在于农产品中。它可通过两步转化为毒性相对较低的3 - 脱氧雪腐镰刀菌烯醇（3 - DON）。已证明ANSP101中的DDH可将DON转化为3 - 酮基 - 脱氧雪腐镰刀菌烯醇（3 - 酮基 - DON）。在本研究中，来自ANSP101的一种依赖NADPH的醛/酮还原酶AKR4被鉴定能够将3 - 酮基 - DON转化为3 - DON。我们的结果表明AKR4显然是一种依赖NADPH的酶，因为它对NADPH的利用率高于NADH。AKR4在pH 5 - 10和温度20 - 60°C的范围内发挥作用。在pH 7和50°C条件下，以NADPH作为辅因子时，AKR4能够在90分钟内降解89%的3 - 酮基 - DON。AKR4作为参与DON降解最后一步的酶的发现，可能为食品和饲料中DON的最终解毒提供一种选择。
