通过顺序自水解和碱性后提取提高麦草的酶水解效率。
Improving enzymatic hydrolysis efficiency of wheat straw through sequential autohydrolysis and alkaline post-extraction.
机构信息
Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China; Materials Science & Engineering College, Nanjing Forestry University, Nanjing 210037, China.
出版信息
Bioresour Technol. 2018 Mar;251:374-380. doi: 10.1016/j.biortech.2017.12.066. Epub 2017 Dec 21.
In this work, a two-step pretreatment process of wheat straw was established by combining autohydrolysis pretreatment and alkaline post-extraction. The results showed that employing alkaline post-extraction to autohydrolyzed wheat straw could significantly improve its enzymatic hydrolysis efficiency from 36.0% to 83.7%. Alkaline post-extraction lead to the changes of the structure characteristics of autohydrolyzed wheat straw. Associations between enzymatic hydrolysis efficiency and structure characteristics were also studied. The results showed that the factors of structure characteristics such as delignification, xylan removal yield, crystallinity, accessibility and hydrophobicity are positively related to enzymatic hydrolysis efficiency within a certain range for alkaline post-extracted wheat straw. The results demonstrated that autohydrolysis coupled with alkaline post-extraction is an effective and promising method to gain fermentable sugars from biomass.
在这项工作中，通过将自水解预处理和碱性后提取相结合，建立了两步预处理小麦秸秆的方法。结果表明，采用碱性后提取对自水解小麦秸秆进行处理，可将其酶解效率从 36.0%显著提高至 83.7%。碱性后提取导致自水解小麦秸秆的结构特性发生变化。同时还研究了酶解效率与结构特性之间的相关性。结果表明，在一定范围内，碱性后提取小麦秸秆的结构特性因素，如脱木质素率、木聚糖去除率、结晶度、可及性和疏水性等，与酶解效率呈正相关。结果表明，自水解与碱性后提取相结合是从生物质中获得可发酵糖的一种有效且有前景的方法。
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