基于正交试验设计的杨树磷酸预处理以优化可发酵糖的生产

Chen Deming, Tang Wenjing, Wang Hui, Sheng Yequan, Tan Xin, Shi Yang, Fan Wei, Ge Shengbo

Ministry of Forestry Bioethanol Research Center, College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, China.

College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui, China.

Front Chem. 2023 Feb 22;11:1119215. doi: 10.3389/fchem.2023.1119215. eCollection 2023.

The recalcitrant structure of raw poplar limited the production of fermentable sugars when applied as the material in the pretreatment of biochemical conversions. Phosphoric acid pretreatment is an efficient method to destroy the compact lignocellulose matrix presence in the poplar. In this study, phosphoric acid pretreatment of poplar was optimised by an orthogonal experimental design [L(3)] to improve enzymatic digestibility through investigating the effects of reaction temperature, time duration, and phosphoric acid concentration. The optimal conditions were selected based on the variance of chemical compositions, hemicellulose removal ratio, and delignification of the woody material after pretreatment. The optimum enzymatic hydrolysis yield of up to 73.44% was obtained when the phosphoric acid pretreatment performed at 190°C for 150 min under 1.5% of v/v phosphoric acid concentration.

当将未加工的杨树用作生物化学转化预处理的原料时，其顽固的结构限制了可发酵糖的产量。磷酸预处理是破坏杨树中存在的致密木质纤维素基质的有效方法。在本研究中，通过正交试验设计[L(3)]对杨树的磷酸预处理进行了优化，通过研究反应温度、持续时间和磷酸浓度的影响来提高酶解消化率。基于预处理后木质材料的化学成分变化、半纤维素去除率和脱木质素情况选择最佳条件。当在190°C、150分钟、1.5%（v/v）磷酸浓度下进行磷酸预处理时，获得了高达73.44%的最佳酶水解产率。