Reece-Hoyes John S, Walhout Albertha J M
Cold Spring Harb Protoc. 2018 Jan 2;2018(1):pdb.top094912. doi: 10.1101/pdb.top094912.
The Gateway recombinatorial cloning system was developed for cloning multiple DNA fragments in parallel (e.g., in 96-well formats) in a standardized manner using the same enzymes. Gateway cloning is based on the highly specific integration and excision reactions of bacteriophage λ into and out of the genome. Because the sites of recombination ("" sites) are much longer (25-242 bp) than restriction sites, they are extremely unlikely to occur by chance in DNA fragments. Therefore, the same recombination enzyme can be used to robustly clone many different fragments of variable size in parallel reactions.
Gateway重组克隆系统是为使用相同的酶以标准化方式并行克隆多个DNA片段（例如，以96孔板形式）而开发的。Gateway克隆基于噬菌体λ基因组的高度特异性整合和切除反应。由于重组位点（“att”位点）比限制酶切位点长得多（25 - 242 bp），它们极不可能偶然出现在DNA片段中。因此，相同的重组酶可用于在平行反应中可靠地克隆许多不同大小的片段。
