Nguyen David, Marchand Paul J, Planchette Arielle L, Nilsson Julia, Sison Miguel, Extermann Jérôme, Lopez Antonio, Sylwestrzak Marcin, Sordet-Dessimoz Jessica, Schmidt-Christensen Anja, Holmberg Dan, Van De Ville Dimitri, Lasser Theo
Laboratoire d'Optique Biomédicale, School of Engineering, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
Autoimmunity, Department of Experimental Medical Sciences, Lund University Diabetes Centre, 20502 Malmö, Sweden.
Biomed Opt Express. 2017 Nov 15;8(12):5637-5650. doi: 10.1364/BOE.8.005637. eCollection 2017 Dec 1.
In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of the intact mouse brain. The system features an isotropic resolution of ~50 µm and an acquisition time of four to eight minutes, using a 3-day optimized clearing protocol. Imaging of the brain autofluorescence in 3D reveals details of the neuroanatomy, while the use of fluorescent labels displays the vascular network and amyloid deposition in 5xFAD mice, an important model of Alzheimer's disease (AD). Finally, the OPT images are compared with histological slices.
近年来，三维介观成像在生命科学中对于全器官水平的基础研究已变得极为重要。在本论文中，我们展示了一种专为完整小鼠脑成像设计的光学投影断层扫描（OPT）方法。该系统具有约50微米的各向同性分辨率以及四到八分钟的采集时间，采用了为期3天的优化透明化方案。对脑自发荧光进行三维成像可揭示神经解剖学细节，而使用荧光标记则能显示5xFAD小鼠（阿尔茨海默病（AD）的重要模型）的血管网络和淀粉样蛋白沉积。最后，将OPT图像与组织切片进行比较。
