Yao Shengkun, Zong Yunbing, Huang Xu, Liu Yang, Gong Ningqiang, Zhang Jianhua, Li Ziqing, Cao Feng, Wang Xiangcheng, Liang Xing-Jie, Jiang Huaidong
School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
These authors contributed equally to this paper.
Biomed Opt Express. 2017 Nov 30;8(12):5825-5833. doi: 10.1364/BOE.8.005825. eCollection 2017 Dec 1.
Cardiovascular diseases are closely related to structural blood capillaries lesions. Herein, microscopic investigations of mouse blood capillaries were performed at multiple spatial resolution by using synchrotron X-ray in-line phase contrast tomography and scanning transmission X-ray microscopy (STXM). The chemically fixed blood capillaries without any contrast agents were selected. For the first time, a periodic bamboo-shaped structure was observed at nanoscale resolution by STXM, and the three-dimensional tomographic slices at sub-micrometer resolution further confirmed the periodic wave profile of the blood capillaries. Then, a periodic microstructural model was suggested based on the microscopic images. By using high-performance imaging techniques, this work provides a better understanding of the relationship between the structure and function of blood capillaries, will be helpful in elucidating the causes of cardiovascular system diseases.
心血管疾病与血管毛细血管结构损伤密切相关。在此，利用同步加速器X射线在线相衬断层扫描和扫描透射X射线显微镜（STXM）在多个空间分辨率下对小鼠毛细血管进行了微观研究。选择了未使用任何造影剂的化学固定毛细血管。首次通过STXM在纳米级分辨率下观察到周期性竹节状结构，亚微米级分辨率的三维断层切片进一步证实了毛细血管的周期性波形轮廓。然后，基于微观图像提出了一个周期性微观结构模型。通过使用高性能成像技术，这项工作为更好地理解毛细血管的结构与功能之间的关系提供了帮助，将有助于阐明心血管系统疾病的病因。
