Tsakanova Gohar, Arakelova Elina, Ayvazyan Violetta, Ayvazyan Anna, Tatikyan Stepan, Aroutiounian Rouben, Dalyan Yeva, Haroutiunian Samvel, Tsakanov Vasili, Arakelyan Arsen
Institute of Molecular Biology of National Academy of Sciences of Republic of Armenia, 7 Hasratyan str., 0014, Yerevan, Armenia.
CANDLE Synchrotron Research Institute, 31 Acharyan str., 0040, Yerevan, Armenia.
Biomed Opt Express. 2017 Nov 30;8(12):5834-5846. doi: 10.1364/BOE.8.005834. eCollection 2017 Dec 1.
Red blood cells (RBCs) are known to be the most suitable cells to study oxidative stress, which is implicated in the etiopathology of many human diseases. The goal of the current study was to develop a new effective approach for assessing oxidative stress in human living RBCs using two-photon microscopy. To mimic oxidative stress in human living RBCs, an model was generated followed by two-photon microscopy imaging. The results revealed that oxidative stress is clearly visible on the two-photon microscopy images of RBCs under oxidative stress compared to no fluorescence in controls (<0.0001). This novel approach for oxidative stress investigation in human living RBCs could efficiently be applied in clinical research and antioxidant compounds testing.
众所周知，红细胞（RBCs）是研究氧化应激最合适的细胞，氧化应激与许多人类疾病的病因病理有关。本研究的目的是开发一种使用双光子显微镜评估人类活红细胞氧化应激的新有效方法。为了模拟人类活红细胞中的氧化应激，构建了一个模型，然后进行双光子显微镜成像。结果显示，与对照组无荧光相比（<0.0001），氧化应激在氧化应激下红细胞的双光子显微镜图像上清晰可见。这种用于研究人类活红细胞氧化应激的新方法可有效地应用于临床研究和抗氧化化合物测试。
