Gilbard J P, Rossi S R, Heyda K G
Cornea Research Unit, Eye Research Institute, Boston, Massachusetts 02114.
Am J Ophthalmol. 1989 Apr 15;107(4):348-55. doi: 10.1016/0002-9394(89)90657-0.
In rabbit studies, we found that extended exposure of the ocular surface to existing ophthalmic solutions resulted in gross surface abnormalities and decreases in conjunctival goblet cell density. We developed an electrolyte solution (solution 15) that preserves normal gross appearance, goblet cell density, corneal epithelial glycogen levels, and ocular surface morphologic characteristics after extended exposure to the rabbit ocular surface. We created an artificial tear formulation by adding a demulcent and a buffering system to solution 15 and reducing its osmolarity to 162 mOsm/l. We then compared our artificial tear formulation to Hypotears in a double-masked, crossover study involving 11 patients with dry-eye disorders. Our artificial tear formulation was more effective than Hypotears in decreasing tear film osmolarity and rose bengal staining, and was preferred subjectively by an eight-to-one margin.
在兔子研究中，我们发现眼表长期暴露于现有的眼科溶液会导致明显的表面异常以及结膜杯状细胞密度降低。我们研发了一种电解质溶液（溶液15），在兔子眼表长期暴露后，该溶液能保持正常的外观、杯状细胞密度、角膜上皮糖原水平以及眼表形态特征。我们通过向溶液15中添加一种润滑剂和一个缓冲系统，并将其渗透压降至162 mOsm/l，从而制成了一种人工泪液制剂。然后，我们在一项涉及11名干眼症患者的双盲交叉研究中，将我们的人工泪液制剂与Hypotears进行了比较。在降低泪膜渗透压和孟加拉玫瑰红染色方面，我们的人工泪液制剂比Hypotears更有效，并且在主观上以八比一的优势更受青睐。
