2nd Faculty of Medicine, Department of Pediatrics, Charles University in Prague and University Hospital Motol, Prague, Czech Republic.
Department of Pediatrics, School of Medicine, University of Jordan, Amman, Jordan.
J Med Virol. 2018 Apr;90(4):767-771. doi: 10.1002/jmv.25012. Epub 2018 Jan 19.
After its computational inference from human stool metagenomes, the CrAssphage has proven to be the most prevalent phage in the human gut, with presumably very wide geographic distribution. The currently available molecular assays do not sufficiently reflect the CrAssphage sequence variability. Here, we report a novel real-time PCR assay whose primers and probes are derived from data of multiple CrAssphage strains obtained from gut viral metagenomes of European, Asian, and African subjects. This assay can be useful in analyses of putative bacterial host co-occurence, and in association studies of non-infectious diseases where the phage may modify the content of gut bacteriomes.
从人类粪便宏基因组的计算推断，CrAssphage 已被证明是人类肠道中最普遍的噬菌体，其地理分布范围可能非常广泛。目前可用的分子检测方法并不能充分反映 CrAssphage 序列的可变性。在这里，我们报告了一种新的实时 PCR 检测方法，其引物和探针源自从欧洲、亚洲和非洲受试者的肠道病毒宏基因组中获得的多个 CrAssphage 菌株的数据。该检测方法可用于分析假定的细菌宿主共同出现，并可用于与噬菌体可能改变肠道细菌组内容的非传染性疾病的关联研究。
