Cervantes-Echeverría Melany, Equihua-Medina Edgar, Cornejo-Granados Fernanda, Hernández-Reyna Abigail, Sánchez Filiberto, López-Contreras Blanca Estela, Canizales-Quinteros Samuel, Ochoa-Leyva Adrián
Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Colonia Chamilpa, 62210, Cuernavaca, Morelos, Mexico.
Unidad de Genómica de Poblaciones Aplicada a la Salud, Facultad de Química, UNAM/Instituto Nacional de Medicina Genómica (INMEGEN), Ciudad de México, México.
BMC Res Notes. 2018 Dec 17;11(1):902. doi: 10.1186/s13104-018-4010-5.
crAssphage is a newly found phage described as the most abundant virus in the human gut microbiome. The majority of the crAssphage proteins are unknown in sequences databases, and its pathogenicity and epidemiology in humans are yet unclear. Hence, being one of the most abundant phages in the human gut microbiome more investigation at the genomic level is necessary to improve our understanding, especially in the Latin American population.
In this article, we provide the whole genome of a crAssphage isolated from the human gut microbiome of the Mexican population, which was named Mexican-crAssphage. The genome consists of 96,283 bp, G+C content of 29.24% and 87 coding sequences. Notably, we did not find any transfer RNA genes in the genome sequence. We also sequenced viral-like enriched particles from 28 fecal samples, and we detected the presence of the Mexican-crAssphage genome in 8 samples (28.5%). To our knowledge, our data is the first whole genome report of the crAssphage isolated from the Latin American Population and provides valuable information for the experimental characterization of the most abundant human gut bacteriophage. The whole genome shotgun project of the Mexican-crAssphage is available at DDBJ/ENA/GenBank under the GenBank MK069403.
crAssphage是一种新发现的噬菌体，被描述为人类肠道微生物群中最丰富的病毒。crAssphage的大多数蛋白质在序列数据库中未知，其在人类中的致病性和流行病学尚不清楚。因此，作为人类肠道微生物群中最丰富的噬菌体之一，有必要在基因组水平上进行更多研究，以增进我们的了解，特别是在拉丁美洲人群中。
在本文中，我们提供了从墨西哥人群的人类肠道微生物群中分离出的一种crAssphage的全基因组，它被命名为墨西哥-crAssphage。该基因组由96,283个碱基对组成，G+C含量为29.24%，有87个编码序列。值得注意的是，我们在基因组序列中未发现任何转运RNA基因。我们还对来自28份粪便样本的病毒样富集颗粒进行了测序，在8个样本（28.5%）中检测到了墨西哥-crAssphage基因组的存在。据我们所知，我们的数据是从拉丁美洲人群中分离出的crAssphage的首个全基因组报告，为这种最丰富的人类肠道噬菌体的实验表征提供了有价值的信息。墨西哥-crAssphage的全基因组鸟枪法测序项目可在DDBJ/ENA/GenBank下获取，登录号为GenBank MK069403。
