慢性牙周炎中基质金属蛋白酶-9和金属蛋白酶组织抑制因子-1启动子甲基化的定量评估
Quantitative Evaluation of MMP-9 and TIMP-1 Promoter Methylation in Chronic Periodontitis.
作者信息
Li Xiting, Lu Jiaxuan, Teng Wei, Zhao Chuanjiang, Ye Xiaolei
机构信息
1 Department of Periodontology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University , Guangzhou, People's Republic of China .
2 Department of Regenerative Medicine, Guangdong Provincial Key Laboratory of Stomatology , Guangzhou, People's Republic of China .
出版信息
DNA Cell Biol. 2018 Mar;37(3):168-173. doi: 10.1089/dna.2017.3948. Epub 2018 Jan 3.
In this study, we investigated the promoter DNA methylation (DNAm) status of the MMP-9 and TIMP-1 genes in patients with chronic periodontitis to evaluate disease progression. Using pyrosequencing technology, DNAm levels of MMP-9 and TIMP-1 CpG islands were measured in 88 chronic periodontitis patients and 15 healthy controls. We found a positive correlation between methylation levels of MMP-9 CpG islands and the severity of chronic periodontitis. Methylated CpG islands were also closely associated with the duration of chronic periodontitis. Moreover, female patients exhibited lower methylation levels of MMP-9 but higher methylation levels of TIMP-1 compared with male patients, and the methylation levels of TIMP-1 gradually decreased with age. The findings of gender disparity in the DNAm of MMP-9 and TIMP-1 genes provide novel insights into chronic periodontitis.
在本研究中，我们调查了慢性牙周炎患者中基质金属蛋白酶-9（MMP-9）和金属蛋白酶组织抑制因子-1（TIMP-1）基因的启动子DNA甲基化（DNAm）状态，以评估疾病进展。使用焦磷酸测序技术，测量了88例慢性牙周炎患者和15例健康对照者中MMP-9和TIMP-1 CpG岛的DNAm水平。我们发现MMP-9 CpG岛的甲基化水平与慢性牙周炎的严重程度呈正相关。甲基化的CpG岛也与慢性牙周炎的病程密切相关。此外，与男性患者相比，女性患者MMP-9的甲基化水平较低，但TIMP-1的甲基化水平较高，且TIMP-1的甲基化水平随年龄增长逐渐降低。MMP-9和TIMP-1基因DNAm中的性别差异研究结果为慢性牙周炎提供了新的见解。
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