Zheng Weiwei, Zhao Junwei, Li Dongmei, Jiang Hao, Han Liyuan, Zhao Xueli, Chen Yufei, Wang Xiangjing, Xiang Wensheng
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
Antonie Van Leeuwenhoek. 2018 Jul;111(7):1095-1103. doi: 10.1007/s10482-017-1012-6. Epub 2018 Jan 3.
Two novel bacterial strains were isolated from the roots of tomato plants (Solanum lycopersicum L.) from Dengfeng, Henan Province, China. Phylogenetic analysis of 16S rRNA gene sequences showed that the isolates NEAU-DE8(1) and NEAU-HE1(2) are closely related to one another (99.6% similarity) and are closely relate to Nonomuraea zeae NEAU-ND5 (98.3, 98.3%) and Nonomuraea gerenzanensis DSM 100948 (98.3, 98.0%). Phylogenetic trees constructed based on 16S rRNA gene sequences revealed that both strains grouped with N. zeae NEAU-ND5 within the clade comprising the members of the genus Nonomuraea. Moreover, key chemotaxonomic properties including major menaquinones, fatty acid composition and phospholipid profiles also confirmed the affiliation of the two strains to the genus Nonomuraea. However, DNA-DNA hybridization data, together with physiological and morphological properties, showed that two isolates could be readily distinguished from their close phylogenetic relatives. Thus, on the basis of these phenotypic and genotypic data, strains NEAU-DE8(1) and NEAU-HE1(2) are concluded to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea lycopersici sp. nov. is proposed. The type strain is NEAU-DE8(1) (= CGMCC 4.7412 = DSM 104642).
从中国河南省登封市番茄植株（Solanum lycopersicum L.）的根部分离出两株新型细菌菌株。对16S rRNA基因序列进行系统发育分析表明，分离株NEAU - DE8(1)和NEAU - HE1(2)彼此密切相关（相似度为99.6%），并且与玉蜀黍野野村菌NEAU - ND5（98.3%、98.3%）和热泉野野村菌DSM 100948（98.3%、98.0%）密切相关。基于16S rRNA基因序列构建的系统发育树显示，这两个菌株在包含野野村菌属成员的进化枝内与玉蜀黍野野村菌NEAU - ND5归为一类。此外，包括主要甲基萘醌、脂肪酸组成和磷脂谱在内的关键化学分类学特性也证实了这两个菌株属于野野村菌属。然而，DNA - DNA杂交数据以及生理和形态学特性表明，这两个分离株可以很容易地与其近缘系统发育亲属区分开来。因此，基于这些表型和基因型数据，得出结论：菌株NEAU - DE8(1)和NEAU - HE1(2)代表野野村菌属的一个新物种，为此提出名为番茄野野村菌（Nonomuraea lycopersici sp. nov.）。模式菌株为NEAU - DE8(1)（= CGMCC 4.7412 = DSM 104642）。
