Shen Yue, Jia Feiyu, Liu Chongxi, Li Jiansong, Guo Siyu, Zhou Shuyu, Wang Xiangjing, Xiang Wensheng
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2016 Jun;66(6):2259-2264. doi: 10.1099/ijsem.0.001019. Epub 2016 Mar 11.
A novel actinobacterium, designated strain NEAU-ND5T, was isolated from the rhizosphere of corn (Zea mays L.) collected in Heilongjiang Province, north-east China, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain NEAU-ND5T was a member of the genus Nonomuraea, with highest sequence similarities to Nonomuraea jabiensis A4036T (98.29 %), Nonomuraea rosea GW12687T (98.25 %), Nonomuraea candida HMC10T (98.22 %), Nonomuraea rhizophila YIM 67092T (98.04 %) and Nonomuraea kuesteri NRRL B-24325T (98.04 %). Similarities to other type strains of the genus Nonomuraea were lower than 98 %. Morphological and chemotaxonomic properties of strain NEAU-ND5T were also consistent with the description of the genus Nonomuraea. The cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, ribose and madurose. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major menaquinones were MK-9(H4), MK-9(H2) and MK-9(H0). The predominant cellular fatty acids were iso-C16:0 and 10-methyl C17:0. A combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-ND5T was clearly distinguished from its closely related Nonomuraea species. Consequently, it is concluded that strain NEAU-ND5T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea zeae sp. nov. is proposed. The type strain is NEAU-ND5T (=CGMCC 4.7280T=DSM 100528T).
从中国东北黑龙江省采集的玉米（Zea mays L.）根际土壤中分离到一株新的放线菌，命名为菌株NEAU-ND5T，并采用多相分类方法对其进行了表征。16S rRNA基因序列分析表明，菌株NEAU-ND5T属于野野村菌属，与贾氏野野村菌A4036T（98.29%）、玫瑰野野村菌GW12687T（98.25%）、白色野野村菌HMC10T（98.22%）、嗜根野野村菌YIM 67092T（98.04%）和库斯特野野村菌NRRL B-24325T（98.04%）的序列相似性最高。与野野村菌属其他模式菌株的相似性低于98%。菌株NEAU-ND5T的形态和化学分类学特征也与野野村菌属的描述一致。细胞壁含有内消旋二氨基庚二酸，全细胞糖为葡萄糖、核糖和马杜拉糖。磷脂谱由双磷脂酰甘油、磷脂酰单甲基乙醇胺、磷脂酰乙醇胺、羟基磷脂酰单甲基乙醇胺、羟基磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰肌醇甘露糖苷组成。主要的甲基萘醌为MK-9（H4）、MK-9（H2）和MK-9（H0）。主要的细胞脂肪酸为异-C16:0和10-甲基-C17:0。DNA-DNA杂交结果和一些表型特征表明，菌株NEAU-ND5T与其亲缘关系密切的野野村菌属物种明显不同。因此，得出结论，菌株NEAU-ND5T代表野野村菌属的一个新物种，为此提出新名称玉米野野村菌（Nonomuraea zeae sp. nov.）。模式菌株为NEAU-ND5T（=CGMCC 4.7280T=DSM 100528T）。
