Department of Medical Services and Techniques, Vocational School of Health Services, Sinop University, 57000, Sinop, Turkey.
Arch Microbiol. 2021 Jul;203(5):2639-2645. doi: 10.1007/s00203-021-02202-z. Epub 2021 Mar 12.
A novel actinobacterium, designated strain K274, was isolated from soil collected from Zafer Cape (Cape Apostolos Andreas), the easternmost tip of Cyprus on the Karpas peninsula, Magusa, Northern Cyprus, and a polyphasic approach was used for characterization of the strain. The isolate was found to have chemotaxonomic and morphological properties associated with members of the genus Nonomuraea. The strain has the highest similarity to Nonomuraea zeae DSM 100528 with 99.1% similarity value. In the phylogenetic dendogram based on 16S rRNA gene sequence, strain K274 was formed a distinct clade together N. zeae DSM 100528, 'Nonomuraea basaltis' 160415 (98.9% similarity), and 'Nonomuraea lycopersici' NEAU-DE8(1) (98.2% similarity). The genome sequence of strain K274 was 11.5 Mbp in size with a total of 11,848 protein-coding genes and 75 RNA genes. The genomic G + C content of the novel strain was 69.7 mol%. Both average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) results between the strain and phlyogenetic neighbours were well below the threshold value, and the novelty are supported by phenotypic and chemotaxonomic differences. Because of all these, strain K274 represents a novel species in the genus Nonomuraea, for which the name Nonomuraea cypriaca sp. nov. is proposed. The type strain is K274 (= DSM 45718 = KCTC 29095).
一株新的放线菌，命名为 K274 菌株，从塞浦路斯北部的 Magusa 地区 Karpas 半岛的 Zafer 海角（阿波斯托洛斯安德烈亚斯海角）采集的土壤中分离得到，采用多相分类法对该菌株进行了鉴定。该分离株具有与游动放线菌属成员相关的化学分类学和形态学特征。该菌株与 Nonomuraea zeae DSM 100528 的相似性最高，相似性值为 99.1%。在基于 16S rRNA 基因序列的系统发育树中，K274 菌株与 N. zeae DSM 100528、'Nonomuraea basaltis' 160415（98.9%相似性）和 'Nonomuraea lycopersici' NEAU-DE8(1)（98.2%相似性）一起形成了一个独特的分支。K274 菌株的基因组序列大小为 11.5 Mbp，共有 11848 个蛋白编码基因和 75 个 RNA 基因。该新菌株的基因组 G+C 含量为 69.7 mol%。菌株与系统发育上的近缘菌株的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）结果均远低于阈值，表型和化学分类学差异也支持其新颖性。因此，K274 菌株代表游动放线菌属中的一个新种，现命名为 Nonomuraea cypriaca sp. nov.。其模式菌株为 K274（=DSM 45718=KCTC 29095）。
