Department of Biological Sciences, University of Calgary, Calgary, T2N 1N4, Canada.
Department of Biotechnology, Hankyong National University, Anseong, 17579, Republic of Korea.
J Microbiol. 2018 Jan;56(1):24-29. doi: 10.1007/s12275-018-7107-6. Epub 2018 Jan 4.
A taxonomic study was conducted on BR7-21, a bacterial strain isolated from the soil of a ginseng field in Baekdu Mountain. Comparative studies of the 16S rRNA gene sequence showed that the isolate was most closely related to Conexibacter woesei DSM 14684, Solirubrobacter pauli ATCC BAA-492, Patulibacter minatonensis JCM 12834, with 93.8%, 92.4%, and 91.5% sequence similarity, respectively; each genus represented a family in the order Solirubrobacterales. Strain BR7-21 was Gram-reaction positive, non-spore forming, aerobic, non-motile, and short rod-shaped. It grew well on half-strength R2A medium. The G + C content of the genomic DNA was 73.9%. It contained meso-diaminopimelic acid in the cell wall and the major menaquinones were MK-7(H) and MK-8(H). The major fatty acids were summarized as (Cω7c/iso-C 2-OH), iso-C, and C cyclo. On the basis of polyphasic evidence, it was proposed that strain BR7-21 should be placed in a new genus and species, for which the name Baekduia soli gen. nov., sp. nov. was proposed with the type strain BR7-21 (= KCTC 22257T = LMG 24797). The family Baekduiaceae fam. nov. is proposed to encompass the genus Baekduia gen. nov.
对从白头山人参田土壤中分离到的 BR7-21 细菌菌株进行了分类学研究。16S rRNA 基因序列的比较研究表明，该分离株与 Conexibacter woesei DSM 14684、Solirubrobacter pauli ATCC BAA-492、Patulibacter minatonensis JCM 12834 最为密切相关，序列相似性分别为 93.8%、92.4%和 91.5%；每个属都代表了 Solirubrobacterales 目中的一个科。BR7-21 菌株革兰氏反应阳性，不形成孢子，好氧，非运动，短杆状。它在半强度 R2A 培养基上生长良好。基因组 DNA 的 G+C 含量为 73.9%。它的细胞壁含有 meso-二氨基庚二酸，主要的menaquinones 是 MK-7(H)和 MK-8(H)。主要脂肪酸总结为 (Cω7c/iso-C 2-OH)、iso-C 和 C 环。基于多相证据，建议将菌株 BR7-21 置于一个新属和新种中，提议将其命名为 Baekduia soli gen. nov., sp. nov.，其模式菌株为 BR7-21(=KCTC 22257T=LMG 24797)。提议建立一个新的科，名为 Baekduiaceae fam. nov.，以包含 Baekduia gen. nov. 属。
