Siddiqi Muhammad Zubair, Lee Soon Youl, Choi Kang Duk, Im Wan-Taek
Department of Biotechnology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 17579, Republic of Korea.
Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyong National University, 327 Chungang-no, Anseong-si, Kyonggi-do, 17579, Republic of Korea.
Curr Microbiol. 2018 May;75(5):624-629. doi: 10.1007/s00284-017-1426-4. Epub 2017 Dec 30.
A Gram-positive, rod-shaped, non-spore-forming, and aerobic bacterium (Gsoil 137) was isolated from soil of a ginseng field of Pocheon province in South Korea and subjected to a polyphasic approach in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 137 was shown to belong to the family Nocardioidaceae and was closely related to Aeromicrobium ginsengisoli Gsoil 098 (96.7%), Aeromicrobium panaciterrae (96.7%), and Aeromicrobium halocynthiae JCM 15749 (96.6%). Being phylogenetic, it was most closely related to Aeromicrobium halocynthiae JCM 15749. The G+C content of the genomic DNA was 70.3 mol%. The diagnostic diamino acid of the cell wall peptidoglycan was LL-diaminopimelic acid. The predominant menaquinone was menaquinone MK-8 (H) and MK-7 (H4) was a minor compound. The major cellular fatty acids were C, C, C ω9c and summed feature 4 (C ω7c/C iso 2-OH). All these data supported the affiliation of strain Gsoil 137 to the genus Aeromicrobium. The results of physiological and biochemical tests enabled strain Gsoil 137 to be differentiated genotypically and phenotypically from currently known Aeromicrobium species. Therefore, strain Gsoil 137 represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium panacisoli sp. nov. is proposed. The type strain is Gsoil 137 (= KCTC 19130 = DSM 17940 = CCUG 52475).
从韩国抱川市人参田土壤中分离出一株革兰氏阳性、杆状、无芽孢形成的需氧细菌（Gsoil 137），并采用多相分类法来确定其分类地位。基于16S rRNA基因序列相似性，菌株Gsoil 137被证明属于诺卡氏菌科，与参田气微菌Gsoil 098（96.7%）、人参气微菌（96.7%）和海鞘气微菌JCM 15749（96.6%）密切相关。在系统发育上，它与海鞘气微菌JCM 15749关系最为密切。基因组DNA的G+C含量为70.3 mol%。细胞壁肽聚糖的诊断性二氨基氨基酸为LL-二氨基庚二酸。主要的甲基萘醌是甲基萘醌MK-8（H），MK-7（H4）是次要成分。主要的细胞脂肪酸是C、C、C ω9c和总和特征4（C ω7c/C异iso 2-OH）。所有这些数据支持菌株Gsoil 137属于气微菌属。生理和生化测试结果使菌株Gsoil 137在基因型和表型上与目前已知的气微菌物种区分开来。因此，菌株Gsoil 137代表气微菌属的一个新物种，为此提出名称人参气微菌（Aeromicrobium panacisoli sp. nov.）。模式菌株是Gsoil 137（=KCTC 19130 = DSM 17940 = CCUG 52475）。
