Gaus G, Doble K E, Price D A, Greenberg M J, Lee T D, Battelle B A
Biol Bull. 1993 Jun;184(3):322-329. doi: 10.2307/1542450.
Five neuropeptides were isolated from CNS extracts of the horseshoe crab Limulus polyphemus by high pressure liquid chromatography (HPLC). The peptides were identified by radioimmunoassays (RIAs) based on two antisera raised to FMRFamide-related peptides (FaRPs). The purified peptides were analyzed by automated sequencing and mass spectrometry, and the following sequences were obtained: DEGHKMLYFamide, GHSLLHFamide, PDHHMMYFamide, DHGNMLYFamide, and GGRSPSLRLRFamide. The first four peptides are members of a novel family with virtually no relationship to FMRFamide. GGRSPSLRLRFamide, on the basis of structural similarity, becomes the second member of a class of FaRPs known previously only from a peptide isolated from mosquito heads. At least one member of the novel family (GHSLLHFamide) inhibits the isolated heart of Limulus.
通过高压液相色谱法（HPLC）从鲎（Limulus polyphemus）的中枢神经系统提取物中分离出了五种神经肽。基于针对FMRF酰胺相关肽（FaRPs）产生的两种抗血清，通过放射免疫测定法（RIAs）对这些肽进行了鉴定。通过自动测序和质谱分析对纯化的肽进行了分析，获得了以下序列：DEGHKMLYF酰胺、GHSLLHF酰胺、PDHHMMYF酰胺、DHGNMLYF酰胺和GGRSPSLRLRF酰胺。前四种肽是一个新家族的成员，与FMRF酰胺几乎没有关系。基于结构相似性，GGRSPSLRLRF酰胺成为一类FaRPs的第二个成员，这类FaRPs此前仅从一种从蚊子头部分离出的肽中得知。这个新家族的至少一个成员（GHSLLHF酰胺）会抑制鲎的离体心脏。
