College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
College of Life Sciences, Ningxia University, Yinchuan 750021, PR China.
Tree Physiol. 2018 Jun 1;38(6):895-910. doi: 10.1093/treephys/tpx162.
To explore the adaptation mechanisms of Kandelia candel (L.) Druce in response to daily flooding, a large-scale quantitative lysine acetylome was carried out using immunoaffinity enrichment of Lys-acetylated peptides and liquid chromatography linked to tandem mass spectrometry. A total of 1041 lysine acetylation (LysAc) sites, 1021 Lys-acetylated peptides and 617 Lys-acetylated proteins were identified. Six conserved sequence motifs of the LysAc sites, including a new motif KxxxxK, were detected. Among these proteins, 260 were differentially acetylated in response to flooding, which were preferentially predicted to participate in carbon metabolism and photosynthesis pathways based on KEGG pathway category enrichment analysis. Consistently, the transcriptional level of acetyltransferase and the consumption of acetyl-CoA were up-regulated under flooding conditions. Most of physiological parameters and mRNA expression levels related to carbon metabolism and photosynthesis were found to be insignificantly affected by flooding. Taken together, reversible protein LysAc is likely to be a post-translational mechanism contributing to the mangrove K. candel's adaptation to daily flooding.
为了探索海漆（Kandelia candel（L.）Druce）适应日常水淹的适应机制，本研究采用免疫亲和富集赖氨酸乙酰化肽和液相色谱串联质谱联用的方法，进行了大规模的定量赖氨酸乙酰化组学研究。共鉴定到 1041 个赖氨酸乙酰化（LysAc）位点、1021 个赖氨酸乙酰化肽和 617 个赖氨酸乙酰化蛋白。检测到 6 个赖氨酸乙酰化位点的保守序列基序，包括一个新的基序 KxxxxK。在这些蛋白质中，有 260 个在水淹响应中发生差异乙酰化，根据 KEGG 途径分类富集分析，这些蛋白质优先预测参与碳代谢和光合作用途径。一致的是，在水淹条件下，乙酰转移酶的转录水平和乙酰辅酶 A 的消耗被上调。大多数与碳代谢和光合作用相关的生理参数和 mRNA 表达水平不受水淹的显著影响。综上所述，可逆的蛋白质 LysAc 可能是一种翻译后机制，有助于红树林海漆适应日常水淹。
