Chávez-Díaz Mercedes Paulina, Escudero-Rincón María Lorenza, Arce-Estrada Elsa Miriam, Cabrera-Sierra Román
Instituto Politécnico Nacional, Departamento de Ingeniería en Metalurgia y Materiales, UPALM Edificio 7, Mexico City 07738, Mexico.
Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Departamento de Ingeniería de Superficies, Corrosión y Durabilidad, Madrid 28040, Spain.
Materials (Basel). 2017 Dec 30;11(1):21. doi: 10.3390/ma11010021.
Two heat treatments were carried out below (Ti6Al4V) and above (Ti6Al4V) Ti6Al4V beta-phase transformation temperature (980 °C), with the purpose of studying the effect of microstructure on the adhesion and proliferation of fibroblast cells, as well as their electrochemical behavior. These alloys were seeded with 10,000 L929 fibroblast cells and immersed for 7 days in the cell culture at 37 °C, pH 7.40, 5% CO₂ and 100% relative humidity. Cell adhesion was characterized by Scanning Electron Microscopy (SEM) and Electrochemical Impedance Spectroscopy (EIS) techniques. Polygonal and elongated cell morphology was observed independent of Ti6Al4V microstructure. Besides, C, O, P, S, Na and Cl signals were detected by Energy Dispersive X-Ray Spectroscopy (EDX), associated with the synthesis of organic compounds excreted by the cells, including protein adsorption from the medium. In certain areas on Ti6Al4V and Ti6Al4V alloys, cells were agglomerated (island type), likely related to the globular microstructure; meanwhile, larger cellular coverage is shown for Ti6Al4V alloy, forming more than one layer on the surface, where only Ca was recorded. Impedance diagrams showed a similar passive behavior for the different Ti6Al4V alloys, mainly due to TiO₂ overlaying the contribution of the organic compounds excreted by fibroblast cells.
在低于（Ti6Al4V）和高于（Ti6Al4V）Ti6Al4Vβ相转变温度（980°C）的条件下进行了两次热处理，目的是研究微观结构对成纤维细胞粘附和增殖的影响及其电化学行为。将这些合金接种10,000个L929成纤维细胞，并在37°C、pH 7.40、5%CO₂和100%相对湿度的细胞培养环境中浸泡7天。通过扫描电子显微镜（SEM）和电化学阻抗谱（EIS）技术对细胞粘附进行表征。观察到多边形和细长形的细胞形态，与Ti6Al4V的微观结构无关。此外，通过能量色散X射线光谱（EDX）检测到C、O、P、S、Na和Cl信号，这些信号与细胞分泌的有机化合物的合成有关，包括从培养基中吸附的蛋白质。在Ti6Al4V和Ti6Al4V合金的某些区域，细胞聚集（岛状），可能与球状微观结构有关；同时，Ti6Al4V合金显示出更大的细胞覆盖率，在表面形成不止一层，且仅记录到Ca。阻抗图显示不同Ti6Al4V合金具有相似的钝化行为，这主要是由于TiO₂覆盖了成纤维细胞分泌的有机化合物的贡献。
