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高温退火纳米羟基磷灰石与成纤维细胞接触的细胞毒性评估

Cytotoxicity Evaluation of High-Temperature Annealed Nanohydroxyapatite in Contact with Fibroblast Cells.

作者信息

Szymonowicz Maria, Korczynski Mariusz, Dobrzynski Maciej, Zawisza Katarzyna, Mikulewicz Marcin, Karuga-Kuzniewska Ewa, Zywickab Boguslawa, Rybak Zbigniew, Wiglusz Rafal J

机构信息

Department of Experimental Surgery and Biomaterial Research, Wroclaw Medical University, Poniatowskiego 2, 50-326 Wroclaw, Poland.

Department of Environment Hygiene and Animal Welfare, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 38c, 51-630 Wroclaw, Poland.

出版信息

Materials (Basel). 2017 May 27;10(6):590. doi: 10.3390/ma10060590.

DOI:10.3390/ma10060590
PMID:28772950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5553407/
Abstract

Biomaterials are substances manufactured for medical purposes in direct contact with the tissues of organisms. Prior to their use, they are tested to determine their usefulness and safety of application. Hydroxyapatites are used in medicine as a bony complement because of their similarity to the natural apatite therein. Thanks to their bioactivity, biocompatibility, stability and non-toxicity hydroxyapatite are the most commonly used materials in osteoimplantology. The use of materials at the nanoscale in medicine or biology may carry the risk of undesirable effects. The aim of the study was to evaluate the cytotoxic effect of high-temperature annealed nanohydroxyapatites on the L929 murine fibroblasts. Nanohydroxyapatites in powder form were obtained by the wet chemistry method: in the temperature range of 800-1000 °C and used for the study. Based on performed studies evaluating the morphology and fibroblast viability, it was found that nanohydroxyapatites show no cytotoxic effects on the L929 cell line.

摘要

生物材料是为医学目的制造的、与生物体组织直接接触的物质。在使用之前，会对它们进行测试以确定其应用的有效性和安全性。羟基磷灰石因其与其中天然磷灰石的相似性而在医学中用作骨补充剂。由于其生物活性、生物相容性、稳定性和无毒性，羟基磷灰石是骨植入学中最常用的材料。在医学或生物学中使用纳米级材料可能存在不良影响的风险。该研究的目的是评估高温退火纳米羟基磷灰石对L929小鼠成纤维细胞的细胞毒性作用。通过湿化学方法获得粉末形式的纳米羟基磷灰石：在800-1000°C的温度范围内，并用于该研究。基于对形态和成纤维细胞活力进行的研究，发现纳米羟基磷灰石对L929细胞系没有细胞毒性作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bb3/5553407/949a0ceec80d/materials-10-00590-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bb3/5553407/c794e6ef7e8c/materials-10-00590-g007a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bb3/5553407/949a0ceec80d/materials-10-00590-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bb3/5553407/01fc56e564a2/materials-10-00590-g005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bb3/5553407/949a0ceec80d/materials-10-00590-g008.jpg

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