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成骨细胞对热处理的Ti6Al4V合金的细胞反应。

Osteoblast Cell Response on the Ti6Al4V Alloy Heat-Treated.

作者信息

Chávez-Díaz Mercedes Paulina, Escudero-Rincón María Lorenza, Arce-Estrada Elsa Miriam, Cabrera-Sierra Román

机构信息

Departamento de Ingeniería en Metalurgia y Materiales, Instituto Politécnico Nacional (ESIQIE-IPN), UPALM Zacatenco, Ciudad de México 07738, Mexico.

Departamento de Ingeniería de Superficies, Corrosión y Durabilidad, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Madrid 28040, Spain.

出版信息

Materials (Basel). 2017 Apr 23;10(4):445. doi: 10.3390/ma10040445.

DOI:10.3390/ma10040445
PMID:28772804
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5506940/
Abstract

In an effort to examine the effect of the microstructural changes of the Ti6Al4V alloy, two heat treatments were carried out below (Ti6Al4V) and above (Ti6Al4V) its β-phase transformation temperature. After each treatment, globular and lamellar microstructures were obtained. Saos-2 pre-osteoblast human osteosarcoma cells were seeded onto Ti6Al4V alloy disks and immersed in cell culture for 7 days. Electrochemical assays in situ were performed using OCP and EIS measurements. Impedance data show a passive behavior for the three Ti6Al4V alloys; additionally, enhanced impedance values were recorded for Ti6Al4V and Ti6Al4V alloys. This passive behavior in culture medium is mostly due to the formation of TiO₂ during their sterilization. Biocompatibility and cell adhesion were characterized using the SEM technique; Ti6Al4V as received and Ti6Al4V alloys exhibited polygonal and elongated morphology, whereas Ti6Al4V alloy displayed a spherical morphology. Ti and O elements were identified by EDX analysis due to the TiO₂ and signals of C, N and O, related to the formation of organic compounds from extracellular matrix. These results suggest that cell adhesion is more likely to occur on TiO₂ formed in discrete α-phase regions (hcp) depending on its microstructure (grains).

摘要

为了研究Ti6Al4V合金微观结构变化的影响，在低于（Ti6Al4V）和高于（Ti6Al4V）其β相变温度的条件下进行了两种热处理。每次处理后，获得了球状和层状微观结构。将Saos-2人成骨前体细胞接种到Ti6Al4V合金圆盘上，并浸入细胞培养液中7天。使用开路电位（OCP）和电化学阻抗谱（EIS）测量进行原位电化学分析。阻抗数据表明三种Ti6Al4V合金呈现出钝态行为；此外，Ti6Al4V和Ti6Al4V合金记录到了增强的阻抗值。在培养基中的这种钝态行为主要归因于它们在灭菌过程中形成了TiO₂。使用扫描电子显微镜（SEM）技术对生物相容性和细胞黏附进行了表征；原始态的Ti6Al4V和Ti6Al4V合金呈现出多边形和拉长的形态，而Ti6Al4V合金呈现出球形形态。通过能谱分析（EDX）鉴定出了Ti和O元素，这是由于TiO₂以及与细胞外基质中有机化合物形成相关的C、N和O信号。这些结果表明，细胞黏附更有可能发生在离散α相区域（密排六方结构）中形成的TiO₂上，这取决于其微观结构（晶粒）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0724/5506940/1bba2ff13436/materials-10-00445-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0724/5506940/1bba2ff13436/materials-10-00445-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0724/5506940/65aaa3ce8e7d/materials-10-00445-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0724/5506940/6653a63695d5/materials-10-00445-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0724/5506940/1bba2ff13436/materials-10-00445-g008.jpg

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