Bethu Murali Satyanarayana, Netala Vasudeva Reddy, Domdi Latha, Tartte Vijaya, Janapala Venkateswara Rao
a Pharmacology and Toxicology Division , CSIR - Indian Institute of Chemical Technology , Hyderabad , India.
b Academy of Scientific and Innovative Research (AcSIR), CSIR - Indian Institute of Chemical Technology , Hyderabad , India.
Artif Cells Nanomed Biotechnol. 2018;46(sup1):104-114. doi: 10.1080/21691401.2017.1414824. Epub 2018 Jan 4.
The present study reports a simple and eco-friendly synthesis of silver nanoparticles (AgNPs) using leaf extract of Rhynchosia suaveolens. UV-Vis analysis of R. suaveolens synthesized AgNPs (RS-AgNPs) showed surface plasmon resonance (SPR) peak at 426 nm. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis revealed that RS-AgNPs were 10-30 nm in size with spherical shape. X-ray diffraction (XRD) analysis of RS-AgNPs confirmed the crystalline nature with face-centered cubic (FCC) lattice. Fourier transform infrared (FTIR) interprets that polyphenols and proteins take part in bioreduction and capping of RS-AgNPs. RS-AgNPs exhibited dose-dependent inhibition of proliferation of different cancer cells including DU145 and PC-3(human prostate carcinoma cell lines), SKOV3 (human ovarian carcinoma) and A549 (human lung adenocarcinoma)with IC values of 4.35, 7.72, 4.2 and 24.7 μg/mL, respectively. The plausible reasons behind anticancer activity of RS-AgNPs were explained using different assays on the most susceptible SKOV3 cells. RS-AgNPs induced oxidative stress in SKOV3 cells by generating reactive oxygen species (ROS), enhancing lipid peroxidation (LPO) levels and decreasing glutathione (GSH) levels. RS-AgNPs induced the apoptosis of SKOV3 cells by up regulating the caspase-3, caspase -8, caspase -9, p53 and BAX and down regulating the antiapoptotic protein Bcl-2. Further, RS-AgNPs showed elevation of caspase 3/7 activity and also exhibited antimigratory effect by inhibiting the migration of SKOV3 cells into the wounded area. The findings suggested that biogenic RS-AgNPs provide an alternative approach to overcome several limitations of chemotherapy.
本研究报道了一种利用光滑鹿藿叶提取物简单且环保地合成银纳米颗粒（AgNPs）的方法。对光滑鹿藿合成的AgNPs（RS-AgNPs）进行紫外可见分析，结果显示在426nm处有表面等离子体共振（SPR）峰。扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析表明，RS-AgNPs尺寸为10 - 30nm，呈球形。对RS-AgNPs进行X射线衍射（XRD）分析，证实其具有面心立方（FCC）晶格的晶体性质。傅里叶变换红外光谱（FTIR）表明，多酚和蛋白质参与了RS-AgNPs的生物还原和包覆过程。RS-AgNPs对包括DU145和PC-3（人前列腺癌细胞系）、SKOV3（人卵巢癌细胞）和A549（人肺腺癌细胞）在内的不同癌细胞的增殖表现出剂量依赖性抑制作用，其IC值分别为4.35、7.72、4.2和24.7μg/mL。通过对最敏感的SKOV3细胞进行不同检测，解释了RS-AgNPs抗癌活性背后的可能原因。RS-AgNPs通过产生活性氧（ROS）、提高脂质过氧化（LPO）水平和降低谷胱甘肽（GSH）水平，在SKOV3细胞中诱导氧化应激。RS-AgNPs通过上调半胱天冬酶-3、半胱天冬酶-8、半胱天冬酶-9、p53和BAX以及下调抗凋亡蛋白Bcl-2，诱导SKOV3细胞凋亡。此外，RS-AgNPs显示出半胱天冬酶3/7活性升高，并且通过抑制SKOV3细胞向损伤区域的迁移还表现出抗迁移作用。研究结果表明，生物合成的RS-AgNPs为克服化疗的若干局限性提供了一种替代方法。
