Li Meng, Li Xiao-Juan, Lü Jian-Hua, Huo Ming-Fei
Collaborative Innovative Center of Food Production and Safety of Henan Province, School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, China.
School of Food Science and Technology, Engineering Research Center of Grain Storage and Security of Ministry of Education, Grain Storage and Logistics National Engineering Laboratory, Henan University of Technology, Lianhua Street, Zhengzhou High-Tech Development Zone, Zhengzhou 450001, Henan, China.
J Therm Biol. 2018 Jan;71:153-157. doi: 10.1016/j.jtherbio.2017.11.007. Epub 2017 Nov 16.
Lasioderma serricorne (Fabricius) (Coleoptera: Anobiidae) is an important economic insect of various stored products worldwide. With the restricted use of methyl bromide and the increasing negative effects of repeated use of phosphine fumigation, heat treatment becomes the main approach to control stored-product insects. We investigated effect of the acclimation to sublethal high temperature of 36°C and 42°C for different times on heat tolerance of L. serricorne adults, pupae, larvae, and eggs to lethal high temperature of 50°C in laboratory. The acclimation of L. serricorne pupae, larvae, and eggs, but not adults, to 36°C improved their survival when exposed to lethal high temperature of 50°C. The acclimation of all life stages of L. serricorne to 42°C significantly improved their survival when exposed to 50°C. For eggs, larvae, and adults, the protective effectiveness of acclimation to 42°C was much more profound than that of to 36°C. LT and LT of all life stages increased with increasing acclimation time to 42°C in general. The LT of L. serricorne adults, pupae, larvae, and eggs acclimation to 42°C for 20h were 2.2, 2.2, 3.4, and 4.8 times higher than that of insects without acclimation, respectively. The results suggest that acclimation to sublethal high temperatures can significantly improve the heat tolerance of L. serricorne by the means of increasing their survival when confronting lethal high temperatures. In the face of global warming, the protective effects induced by acclimation to sublethal high temperatures should be fully considered when design or apply heat treatment to control L. serricorne.
烟草甲（Lasioderma serricorne (Fabricius)，鞘翅目：窃蠹科）是全球各类储藏产品中的一种重要经济昆虫。随着溴甲烷的限制使用以及磷化氢熏蒸反复使用的负面影响不断增加，热处理成为控制储藏物害虫的主要方法。我们在实验室中研究了不同时间适应36°C和42°C亚致死高温对烟草甲成虫、蛹、幼虫和卵对50°C致死高温的耐热性影响。烟草甲的蛹、幼虫和卵（而非成虫）适应36°C后，在暴露于50°C致死高温时其存活率提高。烟草甲所有发育阶段适应42°C后，在暴露于50°C时其存活率显著提高。对于卵、幼虫和成虫而言，适应42°C 的保护效果比适应36°C更为显著。一般而言，所有发育阶段的致死时间（LT）随着适应42°C时间增加而增加。适应42°C达20小时的烟草甲成虫、蛹、幼虫和卵的LT分别比未适应的昆虫高2.2倍、2.2倍、3.4倍和4.8倍。结果表明，适应亚致死高温可通过提高面对致死高温时的存活率来显著提高烟草甲的耐热性。面对全球变暖，在设计或应用热处理来防治烟草甲时，应充分考虑适应亚致死高温所产生的保护作用。
