Halder Soma, Sarkar Mrinmoy, Dey Sananda, Kumar Bhunia Sujay, Ranjan Koley Alok, Giri Biplab
Experimental Medicine & Stem Cell Research Laboratory, Department of Physiology, West Bengal State University, Barasat, Kolkata 700126, India.
Experimental Medicine & Stem Cell Research Laboratory, Department of Physiology, West Bengal State University, Barasat, Kolkata 700126, India; Department of Physiology, University of Gour Banga, Mokdumpur, Malda 732103, India.
J Therm Biol. 2018 Jan;71:32-40. doi: 10.1016/j.jtherbio.2017.10.011. Epub 2017 Nov 10.
Ability of red grape juice (RGJ), a known antioxidant, on testis of adult Wister rat to protect from oxidative stress induced damages by heat stress has been investigated in this study. Heat stress was induced maintaining body and testicular temperature at 43°C for 30min/day for 15 days using a hyperthermia induction chamber. Four groups of rats (n=6 per group) comprising of Group-I (control) -kept at 32°C, Group-II -exposed to heat stress alone, Group-III received RGJ (0.8ml/rat/day) alone and Group-IV -exposed to heat stress and received RGJ at same dose. Analysis of blood and testicular tissue exhibited significant reduction in serum testosterone, testicular superoxide dismutase, testicular catalase and testicular glutathione (all p < 0.001); whereas, significant rise in the level of serum corticosteroid, testicular lipid peroxidase and the apoptotic enzyme caspase-3 of testis (all p < 0.001) were observed along with substantial increase in testicular Hsp72 and Hsf-1, and decrease in 17β-HSD3 were noted in heat stressed rats compared to controls. In Group-IV rats, RGJ administration could restore these parameters to normal levels. The signs of retention were clear in Group-IV rats and found to be significantly different as compared to that of the Group-II rats. In testicular histology of rats exposed to heat stress alone revealed remarkable germ cell degeneration and tubular deformations which were prevented by RGJ treatment (Group-IV). The reduced number of sperm level in Group-II also restored in RGJ treatment (Group-IV). The above results indicate that consumption of RGJ may substantially protect testis from heat stress induce dysfunctions.
本研究调查了已知抗氧化剂红葡萄汁（RGJ）对成年Wister大鼠睾丸的作用，以保护其免受热应激诱导的氧化应激损伤。使用热疗诱导箱，将大鼠的体温和睾丸温度维持在43°C，每天30分钟，持续15天，从而诱导热应激。四组大鼠（每组n = 6）包括：第一组（对照组）——饲养在32°C；第二组——仅暴露于热应激；第三组——仅接受RGJ（0.8ml/只/天）；第四组——暴露于热应激并接受相同剂量的RGJ。血液和睾丸组织分析显示，血清睾酮、睾丸超氧化物歧化酶、睾丸过氧化氢酶和睾丸谷胱甘肽均显著降低（所有p < 0.001）；而与对照组相比，热应激大鼠的血清皮质类固醇水平、睾丸脂质过氧化和睾丸凋亡酶caspase-3显著升高（所有p < 0.001），同时睾丸Hsp72和Hsf-1大幅增加，17β-HSD3减少。在第四组大鼠中，给予RGJ可将这些参数恢复到正常水平。第四组大鼠的保留体征明显，与第二组大鼠相比有显著差异。单独暴露于热应激的大鼠睾丸组织学显示明显的生殖细胞变性和管腔变形，而RGJ治疗（第四组）可预防这些情况。第二组精子数量减少的情况在RGJ治疗（第四组）中也得到恢复。上述结果表明，饮用RGJ可能会显著保护睾丸免受热应激诱导的功能障碍。
