Peruzzi Lorenzo, Franzoni Jacopo, Tiburtini Manuel, Abidi Emanuela, Alù Emiliano, Barone Giulio, Bianchi Elisabetta, Cataudella Chiara, Di Iorio Emanuela, Guerrina Maria, Mondello Fabio, Paino Luca, Pentassuglia Mario, Porrovecchio Manuela, Rivieccio Giovanni, Siccardi Eugenia, Stinca Adriano, Tei Alessio, Volanti Virginia, Giacò Antonio
PLANTSEED Lab, Department of Biology, University of Pisa, Pisa, Italy.
Department of Agricultural, Forest and Food Sciences, University of Palermo, Palermo, Italy.
Comp Cytogenet. 2024 Sep 24;18:175-182. doi: 10.3897/compcytogen.18.135172. eCollection 2024.
Within a practical course of cytotaxonomy organized in Pisa (Italy) on February 2024 by the Group for Floristics, Systematics and Evolution of the Italian Botanical Society, we tested whether using image analysis softwares possible biases are still introduced by different observers. We conclude that observer bias selectively applies in possibly overestimating the length of short arms in a karyotype. As a consequence, the parameters most sensitive to these possible errors are CV and CV, and to a less degree M and THL. To achieve more stable results among observers, a still lacking standardized measurement protocol could be helpful.
2024年2月，在意大利比萨由意大利植物学会植物区系、系统学与进化小组组织的细胞分类学实践课程中，我们测试了使用图像分析软件时不同观察者是否仍会引入可能的偏差。我们得出结论，观察者偏差有选择地适用于可能高估核型中短臂的长度。因此，对这些可能误差最敏感的参数是CV和CV，其次是M和THL。为了在观察者之间获得更稳定的结果，一个仍缺乏的标准化测量方案可能会有所帮助。
