Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University , Shanghai 200240, P. R. China.
Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital , Shanghai 200233, China.
J Proteome Res. 2018 Feb 2;17(2):813-821. doi: 10.1021/acs.jproteome.7b00559. Epub 2018 Jan 25.
Different components of Panax ginseng have different properties and medicinal effects. Metabonomics was a prospective approach to analyze the global response of endogenous metabolites to physiological and pathological processes. In this study, an untargeted metabonomics method using GC/TOFMS combined with multivariate statistical techniques was applied to compare entire metabolite differences and the antistress variations among four components of P. ginseng, namely, total ginsenosides (TG), panaxadiol (PD), panaxatriol (PT), and ginseng polysaccharide (PS), in Wistar rats. The results of metabolite analysis showed that numerous urine metabolites involving neurotransmitters, amino acids, organic acids, and gut microbiota metabolites were changed after administration of the four components of P. ginseng, with TG having the least impact on urinary metabolites. The urinary metabolite profiling of these rats exposed to acute combined stress (forced swimming and behavior restriction) demonstrated that the four ginseng components attenuated urine metabolite changes involving gut microbiota metabolites, tricarboxylic acid (TCA) cycle and energy metabolites, and organic acids to different degrees, with TG improving most of the metabolites altered by stress.
人参的不同成分具有不同的性质和药用功效。代谢组学是一种分析内源性代谢物对生理和病理过程的整体反应的前瞻性方法。在这项研究中，采用 GC/TOFMS 结合多元统计技术的非靶向代谢组学方法，比较了四种人参成分（总皂苷、人参二醇、人参三醇和人参多糖）在 Wistar 大鼠体内的整体代谢物差异和抗应激变化。代谢物分析结果表明，人参四种成分给药后，涉及神经递质、氨基酸、有机酸和肠道微生物代谢物的大量尿代谢物发生变化，其中 TG 对尿代谢物的影响最小。这些大鼠暴露于急性联合应激（强迫游泳和行为限制）后的尿代谢物特征表明，四种人参成分在不同程度上减轻了涉及肠道微生物代谢物、三羧酸（TCA）循环和能量代谢物以及有机酸的尿代谢物变化，其中 TG 改善了应激改变的大多数代谢物。
