Wang Hsin-Shih, Tsai Chia-Lung, Chang Pi-Yueh, Chao Angel, Wu Ren-Chin, Chen Shun-Hua, Wang Chin-Jung, Yen Chih-Feng, Lee Yun-Shien, Wang Tzu-Hao
Department of Obstetrics and Gynecology, LinKou Medical Center, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.
Genomic Medicine Research Core Laboratory, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
PLoS One. 2018 Jan 5;13(1):e0190573. doi: 10.1371/journal.pone.0190573. eCollection 2018.
Stress-induced phosphoprotein-1 (STIP1), an adaptor protein that coordinates the functions of HSP70 and HSP90 in protein folding, has been implicated in the development of human gynecologic malignancies. This case-control study investigates STIP1 serum levels and tissue expression in relation to endometriosis/adenomyosis in Taiwanese population. Female patients with surgically confirmed endometriosis/adenomyosis were compared with women free of endometriosis/adenomyosis. Serum STIP1 levels were measured using an enzyme-linked immunosorbent assay and surgical tissues were analyzed by immunohistochemistry. Both epithelial and stromal cells in surgical tissues of endometriosis and adenomyosis expressed STIP1 and MMP-9. Notably, MMP-9 expression was significantly decreased when STIP1 expression was knocked-down. In vitro experiments revealed that STIP1 was capable of binding to the MMP-9 promoter and enhanced its transcriptional expression. The preoperative serum STIP1 levels of patients with endometriosis/adenomyosis were significantly higher than those of the controls. In brief, our data suggest an association between STIP1 levels and endometriosis/adenomyosis.
应激诱导磷蛋白1（STIP1）是一种衔接蛋白，在蛋白质折叠过程中协调HSP70和HSP90的功能，它与人类妇科恶性肿瘤的发生发展有关。本病例对照研究调查了台湾人群中STIP1的血清水平和组织表达与子宫内膜异位症/子宫腺肌病的关系。将手术确诊为子宫内膜异位症/子宫腺肌病的女性患者与无子宫内膜异位症/子宫腺肌病的女性进行比较。采用酶联免疫吸附测定法检测血清STIP1水平，并用免疫组织化学方法分析手术组织。子宫内膜异位症和子宫腺肌病手术组织中的上皮细胞和基质细胞均表达STIP1和MMP-9。值得注意的是，当STIP1表达被敲低时，MMP-9表达显著降低。体外实验表明，STIP1能够与MMP-9启动子结合并增强其转录表达。子宫内膜异位症/子宫腺肌病患者术前血清STIP1水平显著高于对照组。简而言之，我们的数据表明STIP1水平与子宫内膜异位症/子宫腺肌病之间存在关联。
