微小 RNA-30c 通过靶向食管鳞癌细胞中的 SNAI1 发挥肿瘤抑制作用。

MicroRNA-30c functions as a tumor suppressor via targeting SNAI1 in esophageal squamous cell carcinoma.

机构信息

Department of Cardiothoracic Surgery, Zhongda Hospital, Southeast University, Nanjing 210009, China.

Department of Gastroenterology, Zhongda Hospital, Southeast University, Nanjing 210009, China.

出版信息

Biomed Pharmacother. 2018 Feb;98:680-686. doi: 10.1016/j.biopha.2017.12.095. Epub 2018 Jan 4.

DOI:10.1016/j.biopha.2017.12.095

PMID:29304493

Abstract

BACKGROUND

Aberrant expression of miRNAs was involved in tumor initiation, progression and metastasis in multiple cancers. Many kinds of microRNAs in esophageal squamous cell carcinoma (ESCC) have been researched, whereas miR-30c has not been included.

METHODS

Firstly, we explored the expression of miR-30c in ESCC tissue and serum samples and its relations to the survival. To further investigate its effects on ESCC cells, we completed a series of experiments. We detected the effects of ectopic miR-30c expression on the proliferation, migration and invasion of ESCC cells in vitro. We identified the target role of SNAI1 in ESCC using Dual-luciferase reporter assay and western blot assay.

RESULTS

The results showed miR-30c was significant down-regulated in ESCC tissues and cell lines. Clinically, we found lower miR-30c expression was significantly correlated with worse ESCC progression and survival. Also we clarified that miR-30c suppressed cell proliferation, invasion and epithelial to mesenchymal transition (EMT) of ESCC cell lines. What's more, we figured out that miR-30c inhibits ESCC biological behaviors and EMT progress by directly binding to the 3'-UTR of SNAI1.

CONCLUSION

This study provides new insight into the mechanism responsible for the development of human ESCC. Therefore, miR-30c could be a promising biomarker and a therapeutic target for ESCC in the future.

摘要

背景

miRNA 的异常表达参与了多种癌症的肿瘤发生、进展和转移。已经研究了食管鳞状细胞癌（ESCC）中的许多种类的 microRNAs，但 miR-30c 尚未包括在内。

方法

首先，我们探讨了 miR-30c 在 ESCC 组织和血清样本中的表达及其与生存的关系。为了进一步研究其对 ESCC 细胞的影响，我们完成了一系列实验。我们检测了外源性 miR-30c 表达对 ESCC 细胞体外增殖、迁移和侵袭的影响。我们通过双荧光素酶报告基因检测和 Western blot 检测鉴定了 SNAI1 在 ESCC 中的靶作用。

结果

结果表明，miR-30c 在 ESCC 组织和细胞系中显著下调。临床上，我们发现 miR-30c 表达水平较低与 ESCC 进展和生存不良显著相关。此外，我们阐明了 miR-30c 通过直接结合 SNAI1 的 3'-UTR 抑制 ESCC 细胞系的增殖、侵袭和上皮间质转化（EMT）。更重要的是，我们发现 miR-30c 通过直接结合 SNAI1 的 3'-UTR 抑制 ESCC 细胞的生物学行为和 EMT 进展。