Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Medical Microbiology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran.
J Cancer Res Clin Oncol. 2023 Jul;149(7):3149-3160. doi: 10.1007/s00432-022-04123-w. Epub 2022 Jul 25.
Colorectal cancer (CRC) is the third most prevalent cancer and the second significant cause of cancer-associated death worldwide. The microRNA-30 is a substantial member of the miRNA family and plays a vital role in expanding several cancers. This microRNA potentially targets interleukin 6 as an inflammatory cytokine in CRC.
MSCs were isolated and identified from mice bone marrow and then transduced with lentiviruses containing miR-30C. Transfected MSCs were collected to evaluate IL-6 levels, CT-26 cells were also co-cultured with MSCs, and the effect of apoptosis and IL-6 on the supernatant was assessed.
Our result showed the expression of IL-6 mRNA and the level of protein were decreased in the supernatant of miR-30-transduced MSC cells compared to the control group. In addition, the rate of apoptosis was assessed, and the obtained data revealed the induction of apoptosis in CT-26 cells when they are in the vicinity of miR-30c-transduced MSCs.
We demonstrated that downregulation of miR-30c was significantly correlated with CRC progression and survival. So, the present study elucidated the anticancer effects of miR-30c in CRC and presented a novel target for CRC therapy.
结直肠癌（CRC）是全球第三大常见癌症，也是癌症相关死亡的第二大主要原因。microRNA-30 是 miRNA 家族的重要成员，在多种癌症的发展中起着至关重要的作用。这种 microRNA 可能作为 CRC 中的炎症细胞因子靶向白细胞介素 6。
从老鼠骨髓中分离并鉴定间充质干细胞，然后用含有 miR-30C 的慢病毒进行转导。收集转染的间充质干细胞以评估 IL-6 水平，将 CT-26 细胞与间充质干细胞共培养，并评估细胞凋亡和 IL-6 对上清液的影响。
我们的结果表明，与对照组相比，miR-30 转导的间充质干细胞细胞上清液中 IL-6 mRNA 的表达和蛋白水平降低。此外，评估了细胞凋亡率，获得的数据显示当 CT-26 细胞接近 miR-30c 转导的间充质干细胞时，诱导了 CT-26 细胞的凋亡。
我们证明了 miR-30c 的下调与 CRC 的进展和生存显著相关。因此，本研究阐明了 miR-30c 在 CRC 中的抗癌作用，并为 CRC 治疗提供了一个新的靶点。
