不可逆酶抑制动力学数据的分析
Analysis of kinetic data for irreversible enzyme inhibition.
作者信息
Gray P J, Duggleby R G
机构信息
Materials Research Laboratory, Vic., Australia.
出版信息
Biochem J. 1989 Jan 15;257(2):419-24. doi: 10.1042/bj2570419.
Abstract
Many organophosphorus compounds are irreversible inhibitors of acetylcholinesterase. The methods used in the literature to determine the inhibition kinetic constants usually involve either manual determination of the slope at various points along the inhibition progress curve or fitting polynomials to the curve. The present study investigates the use of non-linear-regression analysis to determine the various parameters. A method is suggested that yields accurate values for the inhibition constants under a range of circumstances.
摘要
许多有机磷化合物是乙酰胆碱酯酶的不可逆抑制剂。文献中用于测定抑制动力学常数的方法通常包括手动测定抑制进程曲线各点的斜率，或者将多项式拟合到该曲线上。本研究探讨了使用非线性回归分析来确定各种参数。提出了一种在一系列情况下都能得出准确抑制常数的方法。
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Avidin is a slow-binding inhibitor of pyruvate carboxylase.抗生物素蛋白是丙酮酸羧化酶的一种慢结合抑制剂。
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