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Analysis of kinetic data for irreversible enzyme inhibition.不可逆酶抑制动力学数据的分析
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2
Interactions of organophosphorus and related compounds with cholinesterases, a theoretical study.有机磷及相关化合物与胆碱酯酶的相互作用：一项理论研究
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[Interaction of membrane-bound and solubilized acetylcholinesterase from human and bovine erythrocytes with organophosphorus inhibitors].[人及牛红细胞中膜结合型和可溶性乙酰胆碱酯酶与有机磷抑制剂的相互作用]
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本文引用的文献

1
Avidin is a slow-binding inhibitor of pyruvate carboxylase.抗生物素蛋白是丙酮酸羧化酶的一种慢结合抑制剂。
Biochemistry. 1982 Jul 6;21(14):3364-70. doi: 10.1021/bi00257a018.
2
Determination of the rate constant of enzyme modification by measuring the substrate reaction in the presence of the modifier.通过在修饰剂存在的情况下测量底物反应来测定酶修饰的速率常数。
Biochemistry. 1982 Mar 2;21(5):1028-32. doi: 10.1021/bi00534a031.
3
Kinetics for the inhibition of acetylcholinesterase from the electric eel by some organophosphates and carbamates.某些有机磷酸酯和氨基甲酸酯对电鳗乙酰胆碱酯酶的抑制动力学。
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Regression analysis of nonlinear Arrhenius plots: an empirical model and a computer program.非线性阿累尼乌斯曲线的回归分析：一个经验模型和一个计算机程序。
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5
Recording spectrophotometric method for determination of dissociation and phosphorylation constants for the inhibition of acetylcholinesterase by organophosphates in the presence of substrate.在底物存在的情况下，用于测定有机磷酸酯对乙酰胆碱酯酶抑制作用的解离常数和磷酸化常数的记录分光光度法。
Biochemistry. 1973 Jul 17;12(15):2940-5. doi: 10.1021/bi00739a026.
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Determination of rate constants for the irreversible inhibition of acetylcholine esterase by continuously monitoring the substrate reaction in the presence of the inhibitor.通过在抑制剂存在的情况下连续监测底物反应来测定乙酰胆碱酯酶不可逆抑制的速率常数。
Biochim Biophys Acta. 1986 Mar 28;870(2):185-90. doi: 10.1016/0167-4838(86)90220-7.
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Estimating enzyme kinetic parameters: a computer program for linear regression and non-parametric analysis.估算酶动力学参数：用于线性回归和非参数分析的计算机程序
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The use of the direct linear plot for determining initial velocities.使用直接线性图来确定初始速度。
Biochem J. 1975 Aug;149(2):305-12. doi: 10.1042/bj1490305.
9
Cholinesterase inhibition studies by stopped-flow instrumentation and automated data processing.通过停流仪器和自动数据处理进行的胆碱酯酶抑制研究。
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不可逆酶抑制动力学数据的分析

Analysis of kinetic data for irreversible enzyme inhibition.

作者信息

Gray P J, Duggleby R G

机构信息

Materials Research Laboratory, Vic., Australia.

出版信息

Biochem J. 1989 Jan 15;257(2):419-24. doi: 10.1042/bj2570419.

DOI:10.1042/bj2570419
PMID:2930459
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1135596/
Abstract

Many organophosphorus compounds are irreversible inhibitors of acetylcholinesterase. The methods used in the literature to determine the inhibition kinetic constants usually involve either manual determination of the slope at various points along the inhibition progress curve or fitting polynomials to the curve. The present study investigates the use of non-linear-regression analysis to determine the various parameters. A method is suggested that yields accurate values for the inhibition constants under a range of circumstances.

摘要

许多有机磷化合物是乙酰胆碱酯酶的不可逆抑制剂。文献中用于测定抑制动力学常数的方法通常包括手动测定抑制进程曲线各点的斜率，或者将多项式拟合到该曲线上。本研究探讨了使用非线性回归分析来确定各种参数。提出了一种在一系列情况下都能得出准确抑制常数的方法。