Gray P J, Skinner D R, Benke K K
Materials Research Laboratory, Defence Science and Technology Organization, Ascot Vale, Australia.
Enzyme Protein. 1993;47(3):116-23. doi: 10.1159/000468667.
The kinetic constants for irreversible enzyme inhibition are determined by non-linear least-square regression using a new optimisation technique. An analysis is given of how calculation of the dissociation constant and the unimolecular rate constant is affected both by the inherent error involved in fitting an exponential curve to the plot of product concentration against time, and by the ill-conditioned nature of the equations relating these constants to the parameters of the exponential. The analysis is applied to simulated sets of product concentration curves.
不可逆酶抑制的动力学常数通过使用一种新的优化技术的非线性最小二乘回归来确定。分析了将指数曲线拟合到产物浓度对时间的图中所涉及的固有误差，以及将这些常数与指数参数相关联的方程的病态性质如何影响解离常数和单分子速率常数的计算。该分析应用于模拟的产物浓度曲线集。
