Laboratory of Food Biotechnology, Institute of Food Science and Nutrition, ETH Zurich, Schmelzbergstrasse 7, CH-8092 Zurich, Switzerland.
Centre Suisse de Recherches Scientifiques en Côte d'Ivoire (CSRS), KM 17 route de Dabou, Adiopodoumé Yopougon, Abidjan - 01 B.P. 1303, Abidjan, Cote d'Ivoire.
Syst Appl Microbiol. 2018 Mar;41(2):65-72. doi: 10.1016/j.syapm.2017.11.003. Epub 2017 Dec 21.
Ten bacterial isolates belonging to the genus Vagococcus were obtained from Malian sour milk fènè produced from spontaneously fermented cow milk. However, these isolates could not be assigned to a species upon initial comparative 16S rRNA gene sequence analysis and were therefore further characterized. Rep-PCR fingerprinting of the isolates yielded four strain clusters represented by strains CG-21 (=DSM 21459), 24CA, CM21 and 9H. Sequence identity of the 16S rRNA gene of DSM 21459 to its closest relative species Vagococcus penaei was 97.9%. Among the four rep strain clusters, DSM 21459 and 24CA shared highest 16S rRNA gene sequence identity of 99.6% while CM21 and 9H shared 98.6-98.8% with DSM 21459 and V. penaei CD276. DSM 21459 and 24CA were thus subjected to a polyphasic typing approach. The genome of DSM 21459 featured a G+C content of 34.1mol% for a 2.17-bp chromosome and a 15-kbp plasmid. Average nucleotide identity (ANI) of DSM 21459 to Vagococcus fluvialis bH819, V. penaei CD276 were 72.88%, 72.63%, respectively. DNA-DNA hybridization (DDH) similarities of strain DSM 21459 to other Vagococcus species were <42.0%. ANI and DDH findings strongly supported the 16S rRNA gene phylogenetic tree delineations. The fatty acid patterns of DSM 21459 was palmitic acid (C , 24.5%), oleic acid (C , 32.8%), stearic acid (C , 18.9%). General physiological characterization of DSM 21459 and 24CA were consistent with those of the genus Vagococcus. Strain DSM 21459 and further strains are therefore considered to belong to a novel species, for which the nomenclature Vagococcus teuberi sp. nov. is proposed. The type strain is named CG-21 (=DSM 21459 and LMG 24695).
从马里酸牛奶发酵的牛奶中分离出了 10 株属于 Vagococcus 属的细菌分离株。然而，通过初步比较 16S rRNA 基因序列分析，这些分离株不能被归为一个种，因此进一步进行了特征描述。分离株的重复序列扩增指纹图谱产生了 4 个菌株簇，由菌株 CG-21（=DSM 21459）、24CA、CM21 和 9H 代表。DSM 21459 的 16S rRNA 基因与最接近的亲缘种 Vagococcus penaei 的序列同一性为 97.9%。在这 4 个重复菌株簇中，DSM 21459 和 24CA 的 16S rRNA 基因序列同一性最高，为 99.6%，而 CM21 和 9H 与 DSM 21459 和 V. penaei CD276 的序列同一性为 98.6-98.8%。因此，DSM 21459 和 24CA 进行了多相分型研究。DSM 21459 的基因组中，染色体的 G+C 含量为 34.1mol%，长度为 2.17bp，质粒长度为 15kbp。DSM 21459 与 Vagococcus fluvialis bH819、V. penaei CD276 的平均核苷酸同一性（ANI）分别为 72.88%、72.63%。菌株 DSM 21459 与其他 Vagococcus 种的 DNA-DNA 杂交（DDH）相似度<42.0%。ANI 和 DDH 结果均强烈支持 16S rRNA 基因系统发育树的划分。DSM 21459 的脂肪酸图谱为棕榈酸（C ，24.5%）、油酸（C ，32.8%）、硬脂酸（C ，18.9%）。DSM 21459 和 24CA 的一般生理特征与 Vagococcus 属一致。DSM 21459 株和进一步的菌株被认为属于一个新种，建议命名为 Vagococcus teuberi sp. nov.。该模式株命名为 CG-21（=DSM 21459 和 LMG 24695）。
